Highly sensitive and selective non-enzymatic glucose detection based on indigo carmine/hemin/H2O2 chemiluminescence

被引:24
作者
Fereja, Tadesse Haile [1 ,2 ,3 ]
Kitte, Shimeles Addisu [1 ,4 ]
Zafar, Muhammad Nadeem [1 ,5 ]
Halawa, Mohamed Ibrahim [1 ,2 ,8 ]
Han, Shuang [1 ,6 ]
Zhang, Wei [1 ]
Xu, Guobao [1 ,7 ]
机构
[1] Chinese Acad Sci, Changchun Inst Appl Chem, State Key Lab Electroanalyt Chem, 5625 Renmin St, Changchun 130022, Jilin, Peoples R China
[2] Univ Chinese Acad Sci, Beijing 100049, Peoples R China
[3] Ambo Univ, Coll Med & Hlth Sci, Dept Pharm, POB 19, Ambo, Ethiopia
[4] Jimma Univ, Coll Nat Sci, Dept Chem, POB 378, Jimma, Ethiopia
[5] Univ Gujrat, Dept Chem, Gujrat 50700, Pakistan
[6] Shenyang Univ Chem Technol, Coll Appl Chem, Shenyang 110142, Peoples R China
[7] Univ Sci & Technol China, Hefei, Peoples R China
[8] Univ Mansoura, Fac Pharm, Dept Pharmaceut Analyt Chem, Mansoura 35516, Egypt
基金
中国国家自然科学基金;
关键词
HYDROGEN-PEROXIDE; CARMINE; OXIDATION; DEGRADATION; NANOWIRES; IMMOBILIZATION; NANOSTRUCTURE; OZONE; URINE; BLOOD;
D O I
10.1039/c9an02100k
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
A new chemiluminescence (CL) system, indigo carmine/glucose/hemin/H2O2, has been found and developed for non-enzymatic detection of indigo carmine (IC) and glucose. The CL response increases linearly with IC concentrations from 3.2 mu M to 10 mM and glucose concentrations from 0.06 mu M to 3.5 mM. The detection limits are 1.45 mu M and 15.0 nM for IC and glucose, respectively. This method allows the determination of glucose in blood and urine after simple dilution. The recoveries for the determination of glucose are between 98.5% and 101.0% in blood and between 98.5% and 101.3% in urine. This method shows good sensitivity, selectivity, simplicity, and is low cost, suggesting its promising broad applications.
引用
收藏
页码:1041 / 1046
页数:6
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