The recombinant ACTH(4-10)-GDNF fusion protein and study of its biological activity

被引：0

作者：

Chen, ZY

Zhang, Y

He, C

Lu, CL

Wu, XF

机构：

[1] Second Mil Med Univ, Dept Neurobiol, Shanghai 200433, Peoples R China

[2] Chinese Acad Sci, Shanghai Inst Biochem, Shanghai 200031, Peoples R China

来源：

PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS | 2001年 / 28卷 / 01期

关键词：

adrenocorticotropin (4-10); glial cell line derived neurotrophic factor; chimeric protein; gene expression;

D O I：

暂无

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The chimeric gene of ACTH (4-10) with GDNF was constructed by PCR amplification. The fused gene was inserted into the expression vector pET-28a (+) and expressed in E. coli. with a level of 30% Of the total bacterial proteins. The expressed product was purified by Ni2+-NTA resin, up to 85% purity. The results of activity assays showed that the chimeric protein could significantly promote the survival of spinal cord neurons and had a higher neurotrophic activity than ACTH (4-10) and GDNF respectively.

引用

页码：67 / 71

页数：5

共 12 条

[1] NERVE GROWTH-FACTOR REVISITED [J].

BRADSHAW, RA ;

BLUNDELL, TL ;

LAPATTO, R ;

MCDONALD, NQ ;

MURRAYRUST, J .

TRENDS IN BIOCHEMICAL SCIENCES, 1993, 18 (02) :48-52

[2]

Chen ZY, 1999, ACTA BIOCH BIOPH SIN, V31, P207

[3]

DUCKERS HJ, 1993, REV NEUROSCI, V4, P321

[4] NEUROPEPTIDES IN PERSPECTIVE - THE LAST 10 YEARS [J].

HOKFELT, T .

NEURON, 1991, 7 (06) :867-879

[5] ACTH-RELATED PEPTIDES - RECEPTORS AND SIGNAL-TRANSDUCTION SYSTEMS INVOLVED IN THEIR NEUROTROPHIC AND NEUROPROTECTIVE ACTIONS [J].

HOL, EM ;

GISPEN, WH ;

BAR, PR .

PEPTIDES, 1995, 16 (05) :979-993

[6] Persephin, a novel neurotrophic factor related to GDNF and neurturin [J].

Milbrandt, J ;

de Sauvage, FJ ;

Fahrner, TJ ;

Baloh, RH ;

Leitner, ML ;

Tansey, MG ;

Lampe, PA ;

Heuckeroth, RO ;

Kotzbauer, PT ;

Simburger, KS ;

Golden, JP ;

Davies, JA ;

Vejsada, R ;

Kato, AC ;

Hynes, M ;

Sherman, D ;

Nishimura, M ;

Wang, LC ;

Vandlen, R ;

Moffat, B ;

Klein, RD ;

Poulsen, K ;

Gray, C ;

Garces, A ;

Henderson, CE ;

Phillips, HS ;

Johnson, EM .

NEURON, 1998, 20 (02) :245-253

[7] RAPID COLORIMETRIC ASSAY FOR CELLULAR GROWTH AND SURVIVAL - APPLICATION TO PROLIFERATION AND CYTO-TOXICITY ASSAYS [J].

MOSMANN, T .

JOURNAL OF IMMUNOLOGICAL METHODS, 1983, 65 (1-2) :55-63

[8]

PLANTINGA LC, 1992, PEPTIDES, V13, P1109

[9]

Sambrook J, 1989, Molecular Cloning: A Laboratory Manual, V2nd

[10] Glial cell line-derived neurotrophic factor-dependent RET activation can be mediated by two different cell-surface accessory proteins [J].

Sanicola, M ;

Hession, C ;

Worley, D ;

Carmillo, P ;

Ehrenfels, C ;

Walus, L ;

Robinson, S ;

Jaworski, G ;

Wei, H ;

Tizard, R ;

Whitty, A ;

Pepinsky, RB ;

Cate, RL .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1997, 94 (12) :6238-6243

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