Production of recombinant human apoptosis signal-regulating kinase 1 (ASK1) in Escherichia coli

被引：0
作者：
Volynets, Galyna P. [1 ]
Gorbatiuk, Oksana B. [1 ,2 ]
Kukharenko, Oleksandr P. [1 ]
Usenko, Mariya O. [1 ]
Yarmoluk, Sergiy M. [1 ]
机构：
[1] NAS Ukraine, Inst Mol Biol & Genet, 150 Zabolotnogo St, UA-03680 Kiev, Ukraine
[2] NAMS Ukraine, State Inst Genet & Regenerat Med, Kiev, Ukraine
来源：
PROTEIN EXPRESSION AND PURIFICATION | 2016年 / 126卷
关键词：
ASK1; Apoptosis signal-regulating kinase 1; Recombinant protein; IPTG induction; Auto-induction protocol; THERAPEUTIC TARGET; INHIBITORS; PROTEIN; IDENTIFICATION; DESIGN; INFLAMMATION; PATHWAY; ROLES;
D O I：
10.1016/j.pep.2016.05.016
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Apoptosis signal-regulating kinase 1 (ASK1) is a mediator of the MAPK signaling cascade, which regulates different cellular processes including apoptosis, cell survival, and differentiation. The increased activity of ASK1 is associated with a number of human diseases and this protein kinase is considered as promising therapeutic target. In the present study, the kinase domain of human ASK1 was expressed in Escherichia coli (E. coli) in soluble form. The expression level of ASK1 was around 0.3-0.47 g per 1 L after using auto-induction protocol or IPTG induction. A one-step on column method for the efficient purification of recombinant ASK1 was performed. Our approach yields sufficient amount of recombinant ASK1, which can be used for inhibitor screening assays and different crystallographic studies. (C) 2016 Elsevier Inc. All rights reserved.
引用
收藏
页码：89 / 92
页数：4
相关论文
共 50 条
  • [21] Regulation and function of apoptosis signal-regulating kinase 1 in rheumatoid arthritis
    Nygaard, Gyrid
    Di Paolo, Julie A.
    Hammaker, Deepa
    Boyle, David L.
    Budas, Grant
    Notte, Gregory T.
    Mikaelian, Igor
    Barry, Vivian
    Firestein, Gary S.
    BIOCHEMICAL PHARMACOLOGY, 2018, 151 : 282 - 290
  • [22] Identification of apoptosis signal-regulating kinase 1 (ASK1) inhibitors among the derivatives of benzothiazol-2-yl-3-hydroxy-5-phenyl-1,5-dihydro-pyrrol-2-one
    Starosyla, Sergiy A.
    Volynets, Galyna P.
    Lukashov, Sergiy S.
    Gorbatiuk, Oksana B.
    Golub, Andriy G.
    Bdzhola, Volodymyr G.
    Yarmoluk, Sergiy M.
    BIOORGANIC & MEDICINAL CHEMISTRY, 2015, 23 (10) : 2489 - 2497
  • [23] Inhibition of apoptosis signal-regulating kinase 1 mitigates the pathogenesis of human immunodeficiency virus-associated nephropathy
    Chen, Anqun
    Xu, Jin
    Lai, Han
    D'Agati, Vivette D.
    Guan, Tian-Jun
    Badal, Shawn
    Liles, John
    He, John C.
    Lee, Kyung
    NEPHROLOGY DIALYSIS TRANSPLANTATION, 2021, 36 (03) : 430 - 441
  • [24] Apoptosis Signal-Regulating Kinase 1 (ASK1) is a Novel Master Molecular Switch Controlling Microglia/Macrophage Reactions That Impact Short- and Long-Term Stroke Outcomes
    Shi Yejie
    Zhang Wenting
    Wang Rongrong
    Leak, Rehana K.
    Chen, Jun
    STROKE, 2019, 50
  • [25] Peroxiredoxin 1 suppresses apoptosis via regulation of the apoptosis signal-regulating kinase 1 signaling pathway in human oral leukoplakia
    Zhang, Min
    Niu, Wenwen
    Zhang, Jianfei
    Ge, Lihua
    Yang, Jing
    Sun, Zheng
    Tang, Xiaofei
    ONCOLOGY LETTERS, 2015, 10 (03) : 1841 - 1847
  • [26] Apoptosis signal-regulating kinase 1 exhibits oncogenic activity in pancreatic cancer
    Luo, Youguang
    Gao, Siqi
    Hao, Ziwei
    Yang, Yang
    Xie, Songbo
    Li, Dengwen
    Liu, Min
    Zhou, Jun
    ONCOTARGET, 2016, 7 (46) : 75155 - 75164
  • [27] Rational design of apoptosis signal-regulating kinase 1 inhibitors: Discovering novel structural scaffold
    Volynets, Galyna P.
    Bdzhola, Volodymyr G.
    Golub, Andriy G.
    Synyugin, Anatoliy R.
    Chekanov, Maksym A.
    Kukharenko, Oleksandr P.
    Yarmoluk, Sergiy M.
    EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY, 2013, 61 : 104 - 115
  • [28] Assembly Dynamics and Stoichiometry of the Apoptosis Signal-regulating Kinase (ASK) Signalosome in Response to Electrophile Stress
    Federspiel, Joel D.
    Codreanu, Simona G.
    Palubinsky, Amy M.
    Winland, Ama J.
    Betanzos, Carlos Morales
    McLaughlin, BethAnn
    Liebler, Daniel C.
    MOLECULAR & CELLULAR PROTEOMICS, 2016, 15 (06) : 1947 - 1961
  • [29] Important role of apoptosis signal-regulating kinase 1 in ischemia-induced angiogenesis
    Izumi, Y
    Kim-Mitsuyama, S
    Yoshiyama, M
    Omura, T
    Shiota, M
    Matsuzawa, A
    Yukimura, T
    Murohara, T
    Takeya, M
    Ichijo, H
    Yoshikawa, J
    Iwao, H
    ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY, 2005, 25 (09) : 1877 - 1883
  • [30] Regulation of Anoxic Death in Caenorhabditis elegans by Mammalian Apoptosis Signal-Regulating Kinase (ASK) Family Proteins
    Hayakawa, Teruyuki
    Kato, Kumiko
    Hayakawa, Ryoichi
    Hisamoto, Naoki
    Matsumoto, Kunihiro
    Takeda, Kohsuke
    Ichijo, Hidenori
    GENETICS, 2011, 187 (03) : 785 - 792
12345