Production of recombinant human apoptosis signal-regulating kinase 1 (ASK1) in Escherichia coli

被引：0
|
作者：
Volynets, Galyna P. [1 ]
Gorbatiuk, Oksana B. [1 ,2 ]
Kukharenko, Oleksandr P. [1 ]
Usenko, Mariya O. [1 ]
Yarmoluk, Sergiy M. [1 ]
机构：
[1] NAS Ukraine, Inst Mol Biol & Genet, 150 Zabolotnogo St, UA-03680 Kiev, Ukraine
[2] NAMS Ukraine, State Inst Genet & Regenerat Med, Kiev, Ukraine
来源：
PROTEIN EXPRESSION AND PURIFICATION | 2016年 / 126卷
关键词：
ASK1; Apoptosis signal-regulating kinase 1; Recombinant protein; IPTG induction; Auto-induction protocol; THERAPEUTIC TARGET; INHIBITORS; PROTEIN; IDENTIFICATION; DESIGN; INFLAMMATION; PATHWAY; ROLES;
D O I：
10.1016/j.pep.2016.05.016
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Apoptosis signal-regulating kinase 1 (ASK1) is a mediator of the MAPK signaling cascade, which regulates different cellular processes including apoptosis, cell survival, and differentiation. The increased activity of ASK1 is associated with a number of human diseases and this protein kinase is considered as promising therapeutic target. In the present study, the kinase domain of human ASK1 was expressed in Escherichia coli (E. coli) in soluble form. The expression level of ASK1 was around 0.3-0.47 g per 1 L after using auto-induction protocol or IPTG induction. A one-step on column method for the efficient purification of recombinant ASK1 was performed. Our approach yields sufficient amount of recombinant ASK1, which can be used for inhibitor screening assays and different crystallographic studies. (C) 2016 Elsevier Inc. All rights reserved.
引用
收藏
页码：89 / 92
页数：4
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