Close relationship between G8-serotype bovine and human rotaviruses isolated in Nigeria

被引：32

作者：

Adah, MI

Nagashima, S

Wakuda, M

Taniguchi, K ^{[1
]}

机构：

[1] Fujita Hlth Univ, Sch Med, Dept Virol & Parasitol, Toyoake, Aichi 4701192, Japan

[2] Univ Maiduguri, Dept Vet Med, Maiduguri, Nigeria

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2003年 / 41卷 / 08期

关键词：

D O I：

10.1128/JCM.41.8.3945-3950.2003

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A bovine rotavirus, NGRBg8, isolated from the feces of a calf with diarrhea in Nigeria was characterized by reverse transcription-PCR, nucleotide sequence analysis, and Northern blot hybridization. The nucleotide sequence of the VP7 gene of the strain was most closely related to that of a Nigerian human G8-serotype strain, HMG035 (99.9%). The NSP1 gene of strain NGRBg8 is highly related (99.4%) to that of a Thai G8 bovine strain, A5-10. Northern blot hybridization revealed a high overall genomic relatedness of bovine strain NGRBg8 with human strain HMG035; all 11 RNA segments hybridized to each other. Thus, the results show the close relationship between G8 bovine and human rotaviruses in Nigeria.

引用

页码：3945 / 3950

页数：6

共 44 条

[1] Further characterization of field strains of rotavirus from Nigeria VP4 genotype P6 most frequently identified among symptomatically infected children [J].