Cloning and nucleotide sequence of amidase gene from Pseudomonas putida

被引：12
作者：
Wu, SJ [1 ]
Fallon, RD [1 ]
Payne, MS [1 ]
机构：
[1] Dupont Co, Cent Res & Dev, Newark, DE 19714 USA
来源：
DNA AND CELL BIOLOGY | 1998年 / 17卷 / 10期
关键词：
D O I：
10.1089/dna.1998.17.915
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Amidases are a class of enzymes which convert amides to acids and have potential value in the development of commercial bioprocesses for the production of useful chemicals, A gene encoding an amidase in Pseudomonas putida 5B has been cloned, sequenced, and overexpressed in Escherichia coli, An additional open reading frame (P38K) encoding a putative protein of 38 kDa was found immediately upstream of the amidase gene, This work continues our characterization of a P. putida operon, which now appears to include P38K, amidase, and a stereo-specific nitrile hydratase, This characterization underlies continuing efforts in biocatalyst development.
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收藏
页码：915 / 920
页数：6
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