DNA barcode to trace the development and differentiation of cord blood stem cells

被引：5

作者：

Wang, Mo-Yu ^{[1
,2
]}

Zhou, Yang ^{[1
,2
]}

Lai, Guang-Shun ^{[1
,3
]}

Huang, Qi ^{[1
,2
]}

Cai, Wen-Qi ^{[1
,2
]}

Han, Zi-Wen ^{[1
,2
]}

Wang, Yingying ^{[1
,2
]}

Ma, Zhaowu ^{[1
,2
]}

Wang, Xian-Wang ^{[1
,4
]}

Xiang, Ying ^{[1
,2
]}

Fang, Shu-Xian ^{[5
,6
]}

Peng, Xiao-Chun ^{[1
,7
]}

Xin, Hong-Wu ^{[1
,2
]}

机构：

[1] Yangtze Univ, Sch Basic Med, Ctr Mol Med, Hlth Sci Ctr,Lab Oncol, 1 Nanhuan Rd, Jingzhou 434023, Hubei, Peoples R China

[2] Yangtze Univ, Sch Basic Med, Dept Biochem & Mol Biol, Hlth Sci Ctr, Jingzhou 434023, Hubei, Peoples R China

[3] Peoples Hosp Lianjiang, Dept Digest Med, Lianjiang 524400, Guangdong, Peoples R China

[4] Yangtze Univ, Sch Basic Med, Dept Lab Med, Hlth Sci Ctr, Jingzhou 434023, Hubei, Peoples R China

[5] Affiliated Canc Hosp, State Key Lab Resp Dis, Guangzhou 510095, Guangdong, Peoples R China

[6] Inst Guangzhou Med Univ, Guangzhou 510095, Guangdong, Peoples R China

[7] Yangtze Univ, Hlth Sci Ctr, Sch Basic Med, Dept Pathophysiol, Jingzhou 434023, Hubei, Peoples R China

来源：

MOLECULAR MEDICINE REPORTS | 2021年 / 24卷 / 06期

基金：

中国国家自然科学基金;

关键词：

umbilical cord blood stem cells; development and differentiation; cell Labeling and lineage tracing; DNA barcode; CLONAL DYNAMICS; IN-VIVO; LINEAGE; EXPRESSION; NEURONS;

D O I：

10.3892/mmr.2021.12489

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Umbilical cord blood transplantation was first reported in 1980. Since then, additional research has indicated that umbilical cord blood stem cells (UCBSCs) have various advantages, such as multi-lineage differentiation potential and potent renewal activity, which may be induced to promote their differentiation into a variety of seed cells for tissue engineering and the treatment of clinical and metabolic diseases. Recent studies suggested that UCBSCs are able to differentiate into nerve cells, chondrocytes, hepatocyte-like cells, fat cells and osteoblasts. The culture of UCBSCs has developed from feeder-layer to feeder-free culture systems. The classical techniques of cell labeling and tracing by gene transfection and fluorescent dye and nucleic acid analogs have evolved to DNA barcode technology mediated by transposon/retrovirus, cyclization recombination-recombinase and clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 strategies. DNA barcoding for cell development tracing has advanced to include single cells and single nucleic acid mutations. In the present study, the latest research findings on the development and differentiation, culture techniques and labeling and tracing of UCBSCs are reviewed. The present study may increase the current understanding of UCBSC biology and its clinical applications.

引用

页数：10

共 82 条

[1] A Multiplexed Single-Cell CRISPR Screening Platform Enables Systematic Dissection of the Unfolded Protein Response [J].