Monitoring of canine parvovirus (CPV) strains detected in vaccinated puppies in Brazil

被引：30

作者：

Castro, T. X. ^{[1
]}

Costa, E. M. ^{[1
]}

Leite, J. P. ^{[2
]}

Labarthe, N. V. ^{[3
]}

Cubel Garcia, R. C. N. ^{[1
]}

机构：

[1] Univ Fed Fluminense, Dept Microbiol & Parasitol, Inst Biomed, BR-24210130 Rio De Janeiro, Brazil

[2] Inst Oswaldo Cruz, Lab Virol Comparada, BR-21040360 Rio De Janeiro, Brazil

[3] Fundacao Oswaldo Cruz, Programa Biodiversidade & Saude, BR-21040361 Rio De Janeiro, Brazil

来源：

RESEARCH IN VETERINARY SCIENCE | 2011年 / 90卷 / 02期

关键词：

Canine parvovirus; Enteritis; Vaccine; PCR; Sequence analysis; RIO-DE-JANEIRO; MOLECULAR CHARACTERIZATION; EVOLUTION; TYPE-2; IMMUNIZATION; EMERGENCE; 2C; VARIANTS; STATE; DOGS;

D O I：

10.1016/j.rvsc.2010.06.005

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

The objective of this study was to investigate, by partial sequencing of VP2 protein, the variability of CPV detected in 37 fecal samples collected from vaccinated puppies with enteritis. Laboratorial diagnosis of CPV was confirmed by HA/HI and PCR and, for sequencing analyses, two different regions of the VP2 gene were amplified by PCR. From 1995 to 2004, all strains were characterized as CPV-2a. After that, both CPV-2a and CPV-2b were detected. All CPV-2a showed a non-synonymous mutation in the residue 297 (Ser -> Ala). A synonymous substitution at the AA 574 was also observed in 15/37 samples. Our findings indicate that the cases of vaccine failure are most likely not associated to the mutations detected in the sequenced regions. However, the monitoring of genotyping mutations that led to new CPV strains is essential to determinate if current vaccines will keep providing protection against all new future variants. (C) 2010 Elsevier Ltd. All rights reserved.

引用

页码：336 / 340

页数：5

共 31 条

[1] Genetic analysis of canine parvovirus isolates (CPV-2) from dogs in Italy [J].