Magnetic tweezers for the mechanical research of DNA at the single molecule level

被引：19
作者：
Xin, Qi [1 ]
Li, Peng [2 ]
He, Yuning [1 ]
Shi, Cuiping [1 ]
Qiao, Yiqun [1 ]
Bian, Xiaojun [1 ]
Su, Jing [3 ]
Qiao, Ruirui [4 ]
Zhou, Xinbo [5 ]
Zhong, Jian [1 ,6 ]
机构：
[1] Shanghai Ocean Univ, Lab Qual & Safety Risk Assessment Aquat Prod Stor, Shanghai Engn Res Ctr Aquat Prod Proc & Preservat, Minist Agr,Coll Food Sci & Technol, Shanghai 201306, Peoples R China
[2] Univ Coll Dublin, Sch Chem & Chem Biol, Dublin 4, Ireland
[3] Shanghai Jiao Tong Univ, Sch Pharm, Shanghai 200240, Peoples R China
[4] Monash Univ, Monash Inst Pharmaceut Sci, Parkville, Vic, Australia
[5] Beijing Inst Pharmacol & Toxicol, Lab Comp Aided Drug Design & Discovery, Beijing 100850, Peoples R China
[6] Chinese Acad Sci, Inst Chem, CAS Key Lab Colloid & Interface & Chem Thermodyna, Beijing 100080, Peoples R China
来源：
ANALYTICAL METHODS | 2017年 / 9卷 / 39期
基金：
中国国家自然科学基金;
关键词：
ATOMIC-FORCE MICROSCOPY; DOUBLE-STRANDED DNA; HOMOLOGOUS RECOMBINATION; HELICASE ACTIVITY; CHROMATIN FIBERS; CELL MECHANICS; MECHANOTRANSDUCTION; DYNAMICS; CONDENSATION; SPECTROSCOPY;
D O I：
10.1039/c7ay01495c
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
Biomechanical forces are involved in the physiological function of molecules. Single molecule magnetic tweezers have been developed and used to investigate the mechanical properties of individual biomolecules and their interactions. In this review, we will mainly review the application of magnetic tweezers for the mechanical research of DNA at the single molecule level. Firstly, we will briefly introduce the fundamentals and the categories of the instrumental setups. Secondly, we will summarize and discuss the application of magnetic tweezers for the analysis of the mechanical properties of DNA and the interactions between DNA and different substances such as DNA-modifying enzymes, DNA structural proteins, cations, drugs, and organic chemicals. Finally, the advantages and disadvantages of magnetic tweezers for DNA research are discussed. This work will provide an important guide to the scientists in the field of DNA mechanics.
引用
收藏
页码：5720 / 5730
页数：11
相关论文
共 78 条
  • [1] Analysis of cell mechanics in single vinculin-deficient cells using a magnetic tweezer
    Alenghat, FJ
    Fabry, B
    Tsai, KY
    Goldmann, WH
    Ingber, DE
    [J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2000, 277 (01) : 93 - 99
  • [2] Direct observation of twisting steps during Rad51 polymerization on DNA
    Arata, Hideyuki
    Dupont, Aurelie
    Mine-Hattab, Judith
    Disseau, Ludovic
    Renodon-Corniere, Axelle
    Takahashi, Masayuki
    Viovy, Jean-Louis
    Cappello, Giovanni
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2009, 106 (46) : 19239 - 19244
  • [3] BAE W, 2014, NAT COMMUN, V5
  • [4] Mechanics and Single-Molecule Interrogation of DNA Recombination
    Bell, Jason C.
    Kowalczykowski, Stephen C.
    [J]. ANNUAL REVIEW OF BIOCHEMISTRY, VOL 85, 2016, 85 : 193 - 226
  • [5] Unraveling the physical chemistry and the mixed binding modes of complex DNA ligands by single molecule stretching experiments
    Bernal, W. F. P.
    Silva, E. F.
    Rocha, M. S.
    [J]. RSC ADVANCES, 2016, 6 (107): : 105631 - 105637
  • [6] Role of tension and twist in single-molecule DNA condensation
    Besteman, K.
    Hage, S.
    Dekker, N. H.
    Lemay, S. G.
    [J]. PHYSICAL REVIEW LETTERS, 2007, 98 (05)
  • [7] DNA Twist Stability Changes with Magnesium(2+) Concentration
    Broekmans, Onno D.
    King, Graeme A.
    Stephens, Greg J.
    Wuite, Gijs J. L.
    [J]. PHYSICAL REVIEW LETTERS, 2016, 116 (25)
  • [8] Recent developments in single-molecule DNA mechanics
    Bryant, Zev
    Oberstrass, Florian C.
    Basu, Aakash
    [J]. CURRENT OPINION IN STRUCTURAL BIOLOGY, 2012, 22 (03) : 304 - 312
  • [9] Single-molecule studies of DNA mechanics
    Bustamante, C
    Smith, SB
    Liphardt, J
    Smith, D
    [J]. CURRENT OPINION IN STRUCTURAL BIOLOGY, 2000, 10 (03) : 279 - 285
  • [10] Single molecule approaches to monitor the recognition and resection of double-stranded DNA breaks during homologous recombination
    Carrasco, Carolina
    Dillingham, Mark S.
    Moreno-Herrero, Fernando
    [J]. DNA REPAIR, 2014, 20 : 119 - 129
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