Rootletin forms centriole-associated filaments and functions in centrosome cohesion

被引:259
作者
Bahe, S
Stierhof, YD
Wilkinson, CJ
Leiss, F
Nigg, EA [1 ]
机构
[1] Max Planck Inst Biochem, Dept Cell Biol, D-82152 Martinsried, Germany
[2] Univ Tubingen, Electron Microscopy Unit, Ctr Plant Mol Biol, D-72076 Tubingen, Germany
关键词
D O I
10.1083/jcb.200504107
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
After duplication of the centriole pair during S phase, the centrosome functions as a single microtubule-organizing center until the onset of mitosis, when the duplicated centrosomes separate for bipolar spindle formation. The mechanisms regulating centrosome cohesion and separation during the cell cycle are not well understood. In this study, we analyze the protein rootletin as a candidate centrosome linker component. As shown by immunoelectron microscopy, endogenous rootletin forms striking fibers emanating from the proximal ends of centrioles. Moreover, rootletin interacts with C-Nap1, a protein previously implicated in A centrosome cohesion. Similar to C-Nap1, rootletin is phosphorylated by Nek2 kinase and is displaced from centrosomes at the onset of mitosis. Whereas the overexpression of rootletin results in the formation of extensive fibers, small interfering RNA-mediated depletion of either rootletin or C-Nap1 causes centrosome splitting, suggesting that both proteins contribute to maintaining centrosome cohesion. The ability of rootletin to form centriole-associated fibers suggests a dynamic model for centrosome cohesion based on entangling filaments rather than continuous polymeric linkers.
引用
收藏
页码:27 / 33
页数:7
相关论文
共 28 条
[1]   Proteomic characterization of the human centrosome by protein correlation profiling [J].
Andersen, JS ;
Wilkinson, CJ ;
Mayor, T ;
Mortensen, P ;
Nigg, EA ;
Mann, M .
NATURE, 2003, 426 (6966) :570-574
[2]  
BOMENS M, 1987, CELL MOTIL CYTOSKEL, V8, P238, DOI DOI 10.1002/CM.970080305
[3]   Centrosome composition and microtubule anchoring mechanisms [J].
Bornens, M .
CURRENT OPINION IN CELL BIOLOGY, 2002, 14 (01) :25-34
[4]  
DOXSEY S, 2005, ANN REV CELL DEV BIO
[5]   Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells [J].
Elbashir, SM ;
Harborth, J ;
Lendeckel, W ;
Yalcin, A ;
Weber, K ;
Tuschl, T .
NATURE, 2001, 411 (6836) :494-498
[6]   EVIDENCE FOR AN INVOLVEMENT OF ACTIN IN THE POSITIONING AND MOTILITY OF CENTROSOMES [J].
EUTENEUER, U ;
SCHLIWA, M .
JOURNAL OF CELL BIOLOGY, 1985, 101 (01) :96-103
[7]   Nek2A kinase stimulates centrosome disjunction and is required for formation of bipolar mitotic spindles [J].
Faragher, AJ ;
Fry, AM .
MOLECULAR BIOLOGY OF THE CELL, 2003, 14 (07) :2876-2889
[8]   C-Nap1, a novel centrosomal coiled-coil protein and candidate substrate of the cell cycle-regulated protein kinase Nek2 [J].
Fry, AM ;
Mayor, T ;
Meraldi, P ;
Stierhof, YD ;
Tanaka, K ;
Nigg, EA .
JOURNAL OF CELL BIOLOGY, 1998, 141 (07) :1563-1574
[9]   SUBSTRATE-SPECIFICITY AND CELL-CYCLE REGULATION OF THE NEK2 PROTEIN-KINASE, A POTENTIAL HUMAN HOMOLOG OF THE MITOTIC REGULATOR NIMA OF ASPERGILLUS-NIDULANS [J].
FRY, AM ;
SCHULTZ, SJ ;
BARTEK, J ;
NIGG, EA .
JOURNAL OF BIOLOGICAL CHEMISTRY, 1995, 270 (21) :12899-12905
[10]   A centrosomal function for the human Nek2 protein kinase, a member of the NIMA family of cell cycle regulators [J].
Fry, AM ;
Meraldi, P ;
Nigg, EA .
EMBO JOURNAL, 1998, 17 (02) :470-481