Spray drying of an attenuated live Newcastle disease vaccine virus intended for respiratory mass vaccination of poultry

被引：29

作者：

Corbanie, E. A. ^{[1
]}

Remon, J. P. ^{[1
]}

Van Reeth, K. ^{[2
]}

Landman, W. J. M. ^{[3
]}

van Eck, J. H. H. ^{[4
]}

Vervaet, C. ^{[1
]}

机构：

[1] Univ Ghent, Fac Pharmaceut Sci, Pharmaceut Technol Lab, B-9000 Ghent, Belgium

[2] Univ Ghent, Fac Vet Med, Virol Lab, B-9000 Ghent, Belgium

[3] Anim Hlth Serv GD, Deventer, Netherlands

[4] Univ Utrecht, Fac Vet Med, Dept Farm Anim Hlth, Utrecht, Netherlands

来源：

VACCINE | 2007年 / 25卷 / 49期

关键词：

spray drying; live attenuated vaccine; mass poultry vaccination;

D O I：

10.1016/j.vaccine.2007.09.049

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

A powder vaccine intended for aerosol vaccination of poultry was formulated by spray drying a live attenuated Newcastle disease virus with potential stabilizers (mannitol, trehalose, polyvinylpyrrollidone (PVP), bovine serum albumin (BSA)). Thermodynamic properties, water sorption, particle size distribution, nebulization properties, density and morphology of the powders were evaluated and the virus survival during spray drying and storage was determined by incubation in embryonated eggs and subsequent haemagglutination assay. All powders had a narrow size distribution with a median volume diameter of +/- 30 mu m (suitable for primary respiratory vaccination of chickens) and good aerosolization characteristics. Four amorphous, hygroscopic formulations were produced (trehalose, trehalose-PVP, trehalose-BSA, trehalose-PVP-BSA), where addition of BSA was beneficial for virus survival during production and storage at 6 and 25 degrees C. A crystalline, non-hygroscopic powder (mannitol) had a lower stabilizing capacity during production but maintained the remaining virus titre during storage. In conclusion, the study demonstrates that it is possible to produce a dry powder formulation of an attenuated live vaccine for mass vaccination of poultry in a one-step spray drying process. (C) 2007 Elsevier Ltd. All rights reserved.

引用

页码：8306 / 8317

页数：12

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