The effect of benzothiazolium salt on spruce callus cells

被引：1
|
作者：
Krajnakova, L. [1 ,2 ]
Kakoniova, D. [1 ]
Liskova, D. [1 ]
Hlinkova, E. [3 ]
机构：
[1] Slovak Acad Sci, Inst Chem, Dubravska Cesta 9, Bratislava 84538, Slovakia
[2] NovaMed Policlin, Banska Bystrica, Slovakia
[3] Comenius Univ, Fac Nat Sci, Inst Cell Biol, Bratislava, Slovakia
来源：
PLANT SOIL AND ENVIRONMENT | 2010年 / 56卷 / 10期
关键词：
benzothiazolium salt; callus culture; linear cell polymorphism; Picea abies L. Karst; RASTIM; 30; DKV; ROOTING CUTTINGS; GROWTH; BENZOLINON;
D O I：
10.17221/149/2009-PSE
中图分类号：
S3 [农学（农艺学）];
学科分类号：
0901 ;
摘要：
The effect of 3-(benzyloxycarbonylmethyl)-2-benzothiazolinone (SM-550) on the growth parameters, cell division, and cell polymorphism of spruce callus cells (Picea abies L. Karst) was investigated. These results were compared with callus parameters grown on the medium supplemented with NAA. The highest concentration (1 mmol) of SM-550 stimulated the growth process, as well as cell division, shortened the lag-phase, and had a significant effect on cells polymorphism. Its effect was demonstrated especially on long-term culture (3 subcultures - 84 days). On the other hand, the highest stimulation of growth by SM-550 in 1 mu mol concentration was determined only in the first subculture. SM-550 in the lowest concentration (1 nmol) used was completely unsuitable in the third subculture, the callus was necrotic and resembled to calli growing on the medium without growth hormones.
引用
收藏
页码：463 / 469
页数：7
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