Hormone Orchestrates a Hierarchical Transcriptional Cascade That Regulates Al-Induced De Novo Root Regeneration in Tea Nodal Cutting

The aluminum in acid soils is very rhizotoxic to most plant species, but it is essential for root growth and development in Camellia sinensis. However, the molecular basis of Al-mediated signaling pathways in root regeneration of tea plants is largely unclear. In this study, we profiled the physiological phenotype, transcriptome, and phytohormones in the process using stems treated with Al (0.3 mM) and control (0.02 mM). The anatomical analysis showed that the 0.3 mM Al-treated stem began to develop adventitious root (AR) primordia within 7 days, ARs occurred after 21 days, while the control showed a significant delay. We further found that the expression patterns of many genes involved in the biosynthesis of ZT, ACC, and JA were stimulated by Al on day 3; also, the expression profiles of auxin transporter-related genes were markedly increased under Al during the whole rooting process. Moreover, the expression of these genes was strongly correlated with the accumulation of ZT, ACC, JA, and IAA. CsERFs, CsMYBs, and CsWRKYs transcription factor genes with possible crucial roles in regulating AR regeneration were also uncovered. Our findings suggest that multiple phytohormones and genes related to their biosynthesis form a hierarchical transcriptional cascade during Al-induced de novo root regeneration in tea nodal cuttings.