An Organellar Nα-Acetyltransferase, Naa60, Acetylates Cytosolic N Termini of Transmembrane Proteins and Maintains Golgi Integrity

被引：92

作者：

Aksnes, Henriette ^{[1
]}

Van Damme, Petra ^{[2
,3
]}

Goris, Marianne ^{[1
]}

Starheim, Kristian K. ^{[1
]}

Marie, Michael ^{[1
]}

Stove, Svein Isungset ^{[1
]}

Hoel, Camilla ^{[1
]}

Kalvik, Thomas Vikestad ^{[1
]}

Hole, Kristine ^{[1
,4
]}

Glomnes, Nina ^{[1
,4
]}

Furnes, Clemens ^{[1
]}

Ljostveit, Sonja ^{[1
]}

Ziegler, Mathias ^{[1
]}

Niere, Marc ^{[1
]}

Gevaert, Kris ^{[2
,3
]}

Arnesen, Thomas ^{[1
,5
]}

机构：

[1] Univ Bergen, Dept Mol Biol, N-5020 Bergen, Norway

[2] Univ Ghent VIB, Dept Med Prot Res, B-9000 Ghent, Belgium

[3] Univ Ghent, Dept Biochem, B-9000 Ghent, Belgium

[4] Univ Bergen, Dept Clin Sci, N-5020 Bergen, Norway

[5] Haukeland Hosp, Dept Surg, N-5021 Bergen, Norway

来源：

CELL REPORTS | 2015年 / 10卷 / 08期

基金：

比利时弗兰德研究基金会;

关键词：

SACCHAROMYCES-CEREVISIAE; GTPASE ARL3P; COMPLEX; YEAST; CONSERVATION; MICROTUBULES; ORGANIZATION; INSIGHTS; REQUIRES; SAN;

D O I：

10.1016/j.celrep.2015.01.053

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

N-terminal acetylation is a major and vital protein modification catalyzed by N-terminal acetyltransferases (NATs). NatF, or N alpha-acetyltransferase 60 (Naa60), was recently identified as a NAT in multicellular eukaryotes. Here, we find that Naa60 differs from all other known NATs by its Golgi localization. A new membrane topology assay named PROMPT and a selective membrane permeabilization assay established that Naa60 faces the cytosolic side of intracellular membranes. An Nt-acetylome analysis of NAA60-knockdown cells revealed that Naa60, as opposed to other NATs, specifically acetylates transmembrane proteins and has a preference for N termini facing the cytosol. Moreover, NAA60 knockdown causes Golgi fragmentation, indicating an important role in the maintenance of the Golgi's structural integrity. This work identifies a NAT associated with membranous compartments and establishes N-terminal acetylation as a common modification among transmembrane proteins, a thus-far poorly characterized part of the N-terminal acetylome.

引用

页码：1362 / 1374

页数：13

共 37 条

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