Complete Genome Sequence of Two Deep-Sea Streptomyces Isolates from Madeira Archipelago and Evaluation of Their Biosynthetic Potential

被引：7

作者：

Albuquerque, Pedro ^{[1
,2
]}

Ribeiro, Ines ^{[3
,4
]}

Correia, Sofia ^{[3
]}

Mucha, Ana Paula ^{[3
,5
]}

Tamagnini, Paula ^{[1
,2
,5
]}

Braga-Henriques, Andreia ^{[6
,7
,8
]}

Carvalho, Maria de Fatima ^{[3
,4
]}

Mendes, Marta, V ^{[1
,2
]}

机构：

[1] Univ Porto, i3S Inst Invest & Inovacao Saude, Rua Alfredo Allen 208, P-4200135 Porto, Portugal

[2] Univ Porto, IBMC Inst Biol Mol & Celular, Rua Alfredo Allen 208, P-4200135 Porto, Portugal

[3] Univ Porto, CIIMAR Interdisciplinary Ctr Marine & Environm Re, Terminal Cruzeiros Porto Leixoes, Ave Gen Norton de Matos S-N, P-4450208 Matosinhos, Portugal

[4] Univ Porto, ICBAS Inst Ciencias Biomed Abel Salazar, Rua Jorge Viterbo Ferreira 228, P-4050313 Porto, Portugal

[5] Univ Porto, Fac Ciencias, Dept Biol, Rua Campo Alegre,Edificio FC4, P-4169007 Porto, Portugal

[6] ARDITI Agencia Reg Desenvolvimento Invest Tecnol, OOM Ocean Observ Madeira, P-9020105 Funchal, Portugal

[7] ARDITI Agencia Reg Desenvolvimento Invest Tecnol, MARE Marine & Environm Sci Ctr, P-9020105 Funchal, Portugal

[8] Govt Azores, Reg Secretariat Sea & Fisheries, Reg Directorate Fisheries, Rua Consul Dabney Colonia Alema, P-9900014 Horta, Portugal

来源：

MARINE DRUGS | 2021年 / 19卷 / 11期

关键词：

Streptomyces; deep-sea actinobacteria; de novo assembly; genome mining; natural products; GENE CLUSTERS; POLYKETIDE SYNTHASE; IDENTIFICATION; ANNOTATION; INSIGHTS; PREDICTION; DISCOVERY; REVEALS;

D O I：

10.3390/md19110621

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

The deep-sea constitutes a true unexplored frontier and a potential source of innovative drug scaffolds. Here, we present the genome sequence of two novel marine actinobacterial strains, MA3_2.13 and S07_1.15, isolated from deep-sea samples (sediments and sponge) and collected at Madeira archipelago (NE Atlantic Ocean; Portugal). The de novo assembly of both genomes was achieved using a hybrid strategy that combines short-reads (Illumina) and long-reads (PacBio) sequencing data. Phylogenetic analyses showed that strain MA3_2.13 is a new species of the Streptomyces genus, whereas strain S07_1.15 is closely related to the type strain of Streptomyces xinghaiensis. In silico analysis revealed that the total length of predicted biosynthetic gene clusters (BGCs) accounted for a high percentage of the MA3_2.13 genome, with several potential new metabolites identified. Strain S07_1.15 had, with a few exceptions, a predicted metabolic profile similar to S. xinghaiensis. In this work, we implemented a straightforward approach for generating high-quality genomes of new bacterial isolates and analyse in silico their potential to produce novel NPs. The inclusion of these in silico dereplication steps allows to minimize the rediscovery rates of traditional natural products screening methodologies and expedite the drug discovery process.

引用

页数：15