Estradiol regulates the expression of CD45 splicing isoforms in lymphocytes

被引:7
作者
Zhang, Tianwei [1 ]
Dong, Zhaoxia [1 ]
Cai, Haitao [1 ]
Rong, Ju [2 ]
Su, Zhongjing [1 ]
机构
[1] Shantou Univ, Dept Histol & Embryol, Med Coll, 22 Xinling Rd, Shantou 515041, Guangdong, Peoples R China
[2] Shantou Univ, Dept Med, Affiliated Hosp 1, Med Coll, Shantou 515041, Guangdong, Peoples R China
基金
中国国家自然科学基金;
关键词
Estradiol; CD45; Alternative splicing; DNA methylation; SYSTEMIC-LUPUS-ERYTHEMATOSUS; DNA METHYLATION;
D O I
10.1007/s11033-020-05373-y
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
CD45, a common leukocyte antigen expressed on the surface of all nucleated hematopoietic cells, indicates the developmental stage and functional status of lymphocytes by its alternative splicing isoforms. Estrogen is correlated with the immune activity of lymphocytes and is involved in the sex bias of several human autoimmune diseases, but the effect of estrogen on the expression of the CD45 splicing isoforms remains unknown. In the present study, a potential estrogen response element was identified on the opposite strand of the CD45 gene by bioinformatics software prediction. The results from RT-qPCR results showed that the expression levels of CD45RO isoform and CD45 antisense RNA were increased after the lymphocytes were treated with 10 nM 17beta-estradiol, and this effect of 17beta-estradiol was reversed when the lymphocytes were cotreated with an estrogen receptor antagonist. Moreover, bisulfite sequencing PCR showed that CD45 DNA methylation in lymphocytes was increased after the treatment with 10 nM 17beta-estradiol. In conclusion, estradiol regulated the expression of CD45 in an estrogen receptor-dependent manner and was associated with CD45 antisense RNA and DNA methylation. The results helped elucidate the regulatory mechanism of the expression of CD45 isoforms and the correlation between estrogen levels and immune activity in females.
引用
收藏
页码:3025 / 3030
页数:6
相关论文
共 24 条
[1]   Estrogen induced concentration dependent differential gene expression in human breast cancer (MCF7) cells: Role of transcription factors [J].
Chandrasekharan, Sabarinath ;
Kandasamy, Krishna Kumar ;
Dayalan, Pavithra ;
Ramamurthy, Viraragavan .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2013, 437 (03) :475-481
[2]   Initiation of T cell signaling by CD45 segregation at 'close contacts' [J].
Chang, Veronica T. ;
Fernandes, Ricardo A. ;
Ganzinger, Kristina A. ;
Lee, Steven F. ;
Siebold, Christian ;
McColl, James ;
Jonsson, Peter ;
Palayret, Matthieu ;
Harlos, Karl ;
Coles, Charlotte H. ;
Jones, E. Yvonne ;
Lui, Yuan ;
Huang, Elizabeth ;
Gilbert, Robert J. C. ;
Klenerman, David ;
Aricescu, A. Radu ;
Davis, Simon J. .
NATURE IMMUNOLOGY, 2016, 17 (05) :574-+
[3]   CD45 functions as a signaling gatekeeper in T cells [J].
Courtney, Adam H. ;
Shvets, Alexey A. ;
Lu, Wen ;
Griffante, Gloria ;
Mollenauer, Marianne ;
Horkova, Veronika ;
Lo, Wan-Lin ;
Yu, Steven ;
Stepanek, Ondrej ;
Chakraborty, Arup K. ;
Weiss, Arthur .
SCIENCE SIGNALING, 2019, 12 (604)
[4]   Mathematical modeling identifies potential gene structure determinants of co-transcriptional control of alternative pre-mRNA splicing [J].
Davis-Turak, Jeremy ;
Johnson, Tracy L. ;
Hoffmann, Alexander .
NUCLEIC ACIDS RESEARCH, 2018, 46 (20) :10598-10607
[5]   Estrogen signaling, through estrogen receptor β, regulates DNA methylation and its machinery in male germ line in adult rats [J].
Dumasia, Kushaan ;
Kumar, Anita ;
Deshpande, Sharvari ;
Balasinor, Nafisa H. .
EPIGENETICS, 2017, 12 (06) :476-483
[6]   Epigenetic mechanisms in systemic lupus erythematosus and other autoimmune diseases [J].
Hedrich, Christian M. ;
Tsokos, George C. .
TRENDS IN MOLECULAR MEDICINE, 2011, 17 (12) :714-724
[7]   CD45: A critical regulator of signaling thresholds in immune cells [J].
Hermiston, ML ;
Xu, Z ;
Weiss, A .
ANNUAL REVIEW OF IMMUNOLOGY, 2003, 21 :107-137
[8]   The immune System Is a natural Target for Estrogen Action: Opposing Effects of Estrogen in Two Prototypical Autoimmune Diseases [J].
Khan, Deena ;
Ahmed, S. Ansar .
FRONTIERS IN IMMUNOLOGY, 2016, 6
[9]   Natural antisense transcripts [J].
Khorkova, Olga ;
Myers, Amanda J. ;
Hsiao, Jane ;
Wahlestedt, Claes .
HUMAN MOLECULAR GENETICS, 2014, 23 :R54-R63
[10]   Natural Antisense Transcripts: Molecular Mechanisms and Implications in Breast Cancers [J].
Latge, Guillaume ;
Poulet, Christophe ;
Bours, Vincent ;
Josse, Claire ;
Jerusalem, Guy .
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2018, 19 (01)