TAR cloning and integrated overexpression of 6-demethylchlortetracycline biosynthetic gene cluster in Streptomyces aureofaciens

被引：16

作者：

Wu, Naxin ^{[1
,2
]}

Huang, He ^{[3
]}

Min, Taoling ^{[2
]}

Hu, Haifeng ^{[2
]}

机构：

[1] Fudan Univ, Sch Pharm, Dept Pharmacol, Shanghai 201203, Peoples R China

[2] Shanghai Inst Pharmaceut Ind, Dept Biopharmceut, Shanghai 201203, Peoples R China

[3] Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Plant Physiol & Ecol, Key Lab Synthet Biol, Shanghai 200032, Peoples R China

来源：

ACTA BIOCHIMICA ET BIOPHYSICA SINICA | 2017年 / 49卷 / 12期

基金：

上海市自然科学基金;

关键词：

6-demethylchlortetracycline; Streptomyces aureofaciens; biosynthetic gene cluster; transformation-associated recombination; RECOMBINATION CLONING; SELECTIVE ISOLATION; HETEROLOGOUS EXPRESSION; COMPLEX GENOMES; DNA; BIOLOGY; COLI;

D O I：

10.1093/abbs/gmx110

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

6-Demethylchlortetracycline (6-DCT), a tetracycline antibiotic produced by Streptomyces aureofaciens, is a crucial precursor employed for the semi-synthesis of tigecycline, minocycline, and amadacyclin (PTK 0796). In this study, the 6-DCT biosynthetic gene cluster (BGC) was cloned from genomic DNA of a high 6-DCT-producing strain, S. aureofaciens DM-1, using the transformation-associated recombination method. An extra copy of the 6-DCT BGC was introduced and integrated into the chromosome of S. aureofaciens DM-1. Duplication of the 6-DCT BGC resulted in a maximum increase of the 6-DCT titer by 34%.

引用

页码：1129 / 1134

页数：6

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