Chondroitin sulfate-functionalized polyamidoamine-mediated miR-34a delivery for inhibiting the proliferation and migration of pancreatic cancer

被引：14

作者：

Duan, Yan ^{[1
]}

Xing, Zhen ^{[1
]}

Yang, Jiebing ^{[1
]}

Wang, Yudi ^{[1
]}

Chen, Jiawen ^{[1
]}

Zhang, Yan ^{[1
]}

Shi, Wei ^{[1
]}

Li, Quanshun ^{[1
]}

机构：

[1] Jilin Univ, Sch Life Sci, Minist Educ, Key Lab Mol Enzymol & Engn, Changchun 130012, Peoples R China

来源：

RSC ADVANCES | 2016年 / 6卷 / 75期

关键词：

HYALURONIC-ACID; GENE; POLYETHYLENIMINE; DENDRIMERS; NANOPARTICLES; MICRORNA-34A; METASTASIS; THERAPY; DRUG;

D O I：

10.1039/c6ra15716e

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Herein, chondroitin sulfate-functionalized polyamidoamine (CS-PAMAM) was employed as a carrier in miR-34a delivery, and the inhibition of cell proliferation and migration of pancreatic cancer was systematically evaluated, using human pancreatic carcinoma cell line MiaPaCa-2 as a model. Through confocal laser scanning microscopy, an efficient cellular uptake of CS-PAMAM/miR-34a nanoparticles has been demonstrated to be executed in a CD44-dependent manner. After the successful delivery of miR-34a, the cell proliferation could be obviously inhibited due to the activation of cell apoptosis and cell cycle arrest. Meanwhile, CS-PAMAM-mediated miR-34a delivery could realize the suppression of cell migration elucidated by wound healing and transwell migration assays. Thus, the derivative CS-PAMAM could potentially be used as an effective carrier for miR-34a delivery to achieve the tumor gene therapy.

引用

页码：70870 / 70876

页数：7

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