Sulfated Polysaccharides in the Freshwater Green Macroalga Cladophora surera Not Linked to Salinity Adaptation

被引:14
作者
Arata, Paula X. [1 ]
Alberghina, Josefina [2 ]
Confalonieri, Viviana [2 ]
Errea, Maria I. [3 ]
Estevez, Jose M. [4 ]
Ciancia, Marina [1 ,5 ]
机构
[1] Univ Buenos Aires, Fac Agron, Dept Biol Aplicada & Alimentos, Catedra Quim Biomol, Buenos Aires, DF, Argentina
[2] Univ Buenos Aires, CONICET, Fac Ciencias Exactas & Nat, Dept Ecol Genet & Evoluc,Inst IEGEBA, Buenos Aires, DF, Argentina
[3] Inst Tecnol Buenos Aires, Dept Ingn Quim, Buenos Aires, DF, Argentina
[4] Consejo Nacl Invest Cient & Tecn, IIBBA, Fdn Inst Leloir, Buenos Aires, DF, Argentina
[5] Univ Buenos Aires, CONICET, Ctr Invest Hidratos Carbono CIHIDECAR, Buenos Aires, DF, Argentina
来源
FRONTIERS IN PLANT SCIENCE | 2017年 / 8卷
关键词
green alga; Cladophora; cell walls; sulfated polysaccharides; freshwater environment; ALGA LAMPROTHAMNIUM-PAPULOSUM; CELL-WALLS; MOLECULAR PHYLOGENY; BROWN-ALGAE; RED ALGAE; CHLOROPHYTA; ULVOPHYCEAE; GALACTANS; EVOLUTION; SEAWEED;
D O I
10.3389/fpls.2017.01927
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
The presence of sulfated polysaccharides in cell walls of seaweeds is considered to be a consequence of the physiological adaptation to the high salinity of the marine environment. Recently, it was found that sulfated polysaccharides were present in certain freshwater Cladophora species and some vascular plants. Cladophora (Ulvophyceae, Chlorophyta) is one of the largest genera of green algae that are able to grow in both, seas and freshwater courses. Previous studies carried out on the water-soluble polysaccharides of the marine species C. falklandica established the presence of sulfated xylogalactoarabinans constituted by a backbone of 4-linked beta-L-arabinopyranose units partially sulfated mainly on C3 and also on C2 with partial glycosylation, mostly on C2, with terminal beta-D-xylopyranose or beta-D-galactofuranose units. Besides, minor amounts of 3-, 6-and/or 3,6-linked beta-D-galactan structures, with galactose in the pyranosic form were detected. In this work, the main water soluble cell wall polysaccharides from the freshwater alga Cladophora surera were characterized. It was found that this green alga biosynthesizes sulfated polysaccharides, with a structure similar to those found in marine species of this genus. Calibration of molecular clock with fossil data suggests that colonization of freshwater environments occurred during the Miocene by its ancestor. Therefore, the presence of sulfated polysaccharides in the freshwater green macroalga C. surera could be, in this case, an adaptation to transient desiccation and changes in ionic strength. Retention of sulfated polysaccharides at the cell walls may represent a snapshot of an evolutionary event, and, thus constitutes an excellent model for further studies on the mechanisms of sulfation on cell wall polysaccharides and environmental stress co-evolution.
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页数:10
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