An Ag-TiO2-reduced graphene oxide hybrid film for electrochemical detection of 8-hydroxy-2′-deoxyguanosine as an oxidative DNA damage biomarker

被引：51

作者：

Dhulkefl, Ayad Jirjees ^{[1
]}

Atacan, Keziban ^{[2
]}

Bas, Salih Zeki ^{[1
]}

Ozmen, Mustafa ^{[1
]}

机构：

[1] Selcuk Univ, Dept Chem, TR-42250 Konya, Turkey

[2] Sakarya Univ, Biomed Magnet & Semicond Mat Applicat & Res Ctr B, TR-54187 Sakarya, Turkey

来源：

ANALYTICAL METHODS | 2020年 / 12卷 / 04期

关键词：

CAPILLARY-ELECTROPHORESIS; PHOTOCATALYTIC ACTIVITY; FACILE SYNTHESIS; VISIBLE-LIGHT; SENSOR; 8-HYDROXYDEOXYGUANOSINE; NANOPARTICLES; PERFORMANCE; COMPOSITE; REDUCTION;

D O I：

10.1039/c9ay02175b

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

In the present work, a notably sensitive and selective electrochemical 8-OHdG sensor based on an Ag-TiO2-reduced graphene oxide (rGO) hybrid nanomaterial modified screen-printed electrode (SPE) was developed for 8-hydroxy-2 '-deoxyguanosine (8-OHdG) determination, which is a significant DNA biomarker because it is one of the main products of oxidative DNA damage. The amperometric responses of the fabricated sensor (Ag-TiO2-rGO/SPE) were measured via monitoring the oxidation current of 8-OHdG by using the differential pulse voltammetry (DPV) technique. The effects of the rGO : Ag-TiO2 ratio, amount of Ag-TiO2-rGO on the electrode surface, electrolyte pH, and possible interfering substances on the current response of Ag-TiO2-rGO/SPE were investigated in detail. Under optimized conditions, the current signals linearly correlated with the concentration of 8-OHdG in the range of 0.05-25 mu M (R-2 = 0.9967) with a detection limit of 10 nM. Furthermore, Ag-TiO2-rGO/SPE was successfully tested to determine the 8-OHdG level in a human urine sample (only in a volume of 50 mu L) with satisfactory recovery.

引用

页码：499 / 506

页数：8

共 42 条

[1] CuFe2O4/reduced graphene oxide nanocomposite decorated with gold nanoparticles as a new electrochemical sensor material for L-cysteine detection [J].