Extremely Bright, Near-IR Emitting Spontaneously Blinking Fluorophores Enable Ratiometric Multicolor Nanoscopy in Live Cells

被引：35

作者：

Tyson, Jonathan ^{[1
,2
]}

Hu, Kevin ^{[3
,4
]}

Zheng, Shuai ^{[1
]}

Kidd, Phylicia ^{[3
]}

Dadina, Neville ^{[1
]}

Chu, Ling ^{[2
,3
]}

Toomre, Derek ^{[3
,5
]}

Bewersdorf, Joerg ^{[3
,4
,5
,9
]}

Schepartz, Alanna ^{[1
,2
,6
,7
,8
]}

机构：

[1] Univ Calif Berkeley, Dept Chem, Berkeley, CA 94720 USA

[2] Yale Univ, Dept Chem, New Haven, CT 06520 USA

[3] Yale Sch Med, Dept Cell Biol, New Haven, CT 06510 USA

[4] Yale Univ, Dept Biomed Engn, New Haven, CT 06511 USA

[5] Yale Univ, Nanobiol Inst, West Haven, CT 06516 USA

[6] Univ Calif Berkeley, Dept Chem, Dept Mol & Cellular Biol, Berkeley, CA 94720 USA

[7] Univ Calif Berkeley, Calif Inst Quantitat Biosci QB3, Berkeley, CA 94720 USA

[8] Yale Univ, Dept Mol Cellular & Dev Biol, New Haven, CT 06520 USA

[9] Yale Sch Med, Kavli Inst Neurosci, New Haven, CT 06510 USA

来源：

ACS CENTRAL SCIENCE | 2021年 / 7卷 / 08期

基金：

英国惠康基金;

关键词：

GENERAL-METHOD; INTRAMOLECULAR SPIROCYCLIZATION; SUPERRESOLUTION MICROSCOPY; RHODAMINE; PROBES; DYES;

D O I：

10.1021/acscentsci.1c00670

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

New bright, photostable, emission-orthogonal fluorophores that blink without toxic additives are needed to enable multicolor, live-cell, single-molecule localization microscopy (SMLM). Here we report the design, synthesis, and biological evaluation of Yale(676sb), a photostable, near-IR-emitting fluorophore that achieves these goals in the context of an exceptional quantum yield (0.59). When used alongside HMSiR, Yale(676sb) enables simultaneous, live-cell, two-color SMLM of two intracellular organelles (ER + mitochondria) with only a single laser and no chemical additives.

引用

页码：1419 / 1426

页数：8