Genotyping Neisseria gonorrhoeae gyrA and penA antimicrobial genes from remnant Neisseria gonorrhoeae positive Cepheid Xpert® clinical specimens - A feasibility study

被引：0
|
作者：
Qadir, S. [1 ]
Ellis, O. [2 ]
Keizur, E. M. [1 ]
Stafylis, C. [1 ]
Cortado, R. [3 ]
Klausner, J. D. [1 ]
机构：
[1] Univ Calif Los Angeles, David Geffen Sch Med, Div Infect Dis, Los Angeles, CA 90095 USA
[2] Univ Calif Los Angeles, Fielding Sch Publ Hlth, Dept Environm Hlth Sci, Los Angeles, CA USA
[3] Univ Calif Los Angeles, David Geffen Sch Med, Dept Pediat, Los Angeles, CA 90095 USA
来源：
JOURNAL OF MICROBIOLOGICAL METHODS | 2020年 / 168卷
基金：
美国国家卫生研究院;
关键词：
Genotyping; penA; gyrA; Neisseria gonorrhoeae; Antibiotic resistance; TIME PCR ASSAY; RESISTANCE;
D O I：
10.1016/j.mimet.2019.105783
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Neisseria gonorrhoeae (NG) has developed resistance to most antibiotics, making it increasingly difficult to treat. Previous studies have predicted antimicrobial NG susceptibility based on the antimicrobial gene target DNA gyrase subunit A (gyrA) codon serine 91 and the penicillin-binding protein 2 (penA) using Roche Cobas (R) and Hologic APTIMA (TM) clinical specimens. We studied whether similar methods could be used on remnant NG-positive Cepheid Xpert (R) specimens.
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页数：2
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