Lancemaside A Inhibits Lipopolysaccharide-Induced Inflammation by Taregting LPS/TLR4 Complex

被引:37
作者
Joh, Eun-Ha [1 ]
Kim, Dong-Hyun [1 ]
机构
[1] Kyung Hee Univ, Dept Life & Nanopharmaceut Sci, Seoul 130701, South Korea
关键词
LANCEMASIDE A; TLR4; LPS; IRAK-4; INFLAMMATION; NF-KAPPA-B; GINSENOSIDE RB1; GENE-EXPRESSION; COMPOUND K; NEUTROPHIL EFFEROCYTOSIS; ENDOTHELIAL-CELLS; PLATYCODIN-D; TNF-ALPHA; DISEASE; RECEPTOR;
D O I
10.1002/jcb.22773
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
In our previous study, lancemaside A isolated from Codonopsis lanceolata (family Campanulaceae) ameliorated colitis in mice. In this study, the anti-inflammatory effects of lancemaside A was investigated ill lipopolysaccharide (LPS)-stimulated mice and their peritoneal macrophage cells. Lancemaside A suppressed the production of pro-inflammatory cytokines. TNF-alpha and IL-1 beta, in vitro and in vivo. Lancemaside A also down-regulated inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2), as well as the inflammatory mediators, :nitric oxide (NO), and PGE(2). Lancemaside A also inhibited the expression of IL-1 receptor-associated kinase-4 (IRAK-4), the phosphorylation of IKK-beta and I kappa B-alpha, the nuclear translocation of NF-kappa B and the activation of mitogen-activated protein kinases in LPS-stimulated peritoneal macrophages. Furthermore, lancemaisde A inhibited the interaction between LPS and TLR4, as well as IRAK-4 expression in peritoneal macrophages. Based on these findings, lancemaside A expressed anti-inflammatory effects by regulating both the binding of LPS to TLR4 on macrophages. J. Cell. Biochem. 111: 865-871,2010. (C) 2010 Wiley-Liss, Inc.
引用
收藏
页码:865 / 871
页数:7
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