Background Chronic pulmonary aspergillosis (CPA) is a progressive respiratory disease, caused most commonly by A fumigatus, with significant morbidity and mortality. Azole resistance in A fumigatus is a growing concern worldwide, with resistance to itraconazole reported in up to 50% of patients. Aim The aim of this study was to determine whether a positive Aspergillus PCR (polymerase chain reaction) is a marker of resistance in CPA patients on azole therapy. Methods Patients were selected via a consecutive database search for the first 50 CPA patients with a positive Aspergillus PCR from January to September 2016. Data were collected regarding concurrent and subsequent culture results, current therapy and serum antifungal levels. PCR-positive patients not on therapy were included as the control group. Results Twenty-three patients were on therapy (15 itraconazole, 4 voriconazole and 4 posaconazole). Cycle threshold (Ct) values ranged from 20.8 to 37.9; no significant difference was found between each treatment and the control group (P = .47). In treated patients, concurrent azole-resistant A fumigatus was found in 75% of A fumigatus-positive cultures (6/8). All of the resistant isolates in the itraconazole group showed therapy resistance. Twenty per cent of all itraconazole levels were sub-therapeutic. No significant difference was found in serum itraconazole levels for patients on itraconazole with a positive PCR versus negative PCR (P = .44). Conclusion Positive sputum, Aspergillus-specific PCR can be associated with azole resistance in CPA patients on therapy.
机构:
Inst Salud Carlos III, Natl Ctr Microbiol, Mycol Reference Lab, Madrid, SpainInst Salud Carlos III, Natl Ctr Microbiol, Mycol Reference Lab, Madrid, Spain
Alastruey-Izquierdo, Ana
;
Cadranel, Jacques
论文数: 0引用数: 0
h-index: 0
机构:
Hop Tenon, AP HP, Serv Pneumol, Paris, France
Sorbonne Univ, Paris, FranceInst Salud Carlos III, Natl Ctr Microbiol, Mycol Reference Lab, Madrid, Spain
Cadranel, Jacques
;
Flick, Holger
论文数: 0引用数: 0
h-index: 0
机构:
Med Univ Graz, Div Pulmonol, Dept Internal Med, Graz, AustriaInst Salud Carlos III, Natl Ctr Microbiol, Mycol Reference Lab, Madrid, Spain
机构:
UPMC Univ Paris 06, Sorbonne Univ, CRSA, INSERM, Paris, France
Hop St Antoine, AP HP, Serv Parasitol Mycol, Paris, FranceInst Salud Carlos III, Natl Ctr Microbiol, Mycol Reference Lab, Madrid, Spain
Hennequin, Christophe
;
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机构:
Hoenigl, Martin
;
Kosmidis, Chris
论文数: 0引用数: 0
h-index: 0
机构:
Manchester Univ Fdn Trust, Natl Aspergillosis Ctr, Manchester, Lancs, England
Univ Manchester, Div Infect Immun & Resp Med, Fac Biol Med & Hlth, Manchester Acad Hlth Sci Ctr, Manchester, Lancs, EnglandInst Salud Carlos III, Natl Ctr Microbiol, Mycol Reference Lab, Madrid, Spain
Kosmidis, Chris
;
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机构:
Lange, Christoph
;
Munteanu, Oxana
论文数: 0引用数: 0
h-index: 0
机构:
State Univ Med & Pharm Nicolae Testemitanu, Div Pneumol & Allergol, Dept Internal Med, Kishinev, MoldovaInst Salud Carlos III, Natl Ctr Microbiol, Mycol Reference Lab, Madrid, Spain
Munteanu, Oxana
;
Page, Iain
论文数: 0引用数: 0
h-index: 0
机构:
Clin TB Ctr, German Ctr Infect Res, Borstel, GermanyInst Salud Carlos III, Natl Ctr Microbiol, Mycol Reference Lab, Madrid, Spain
Page, Iain
;
Salzer, Helmut J. F.
论文数: 0引用数: 0
h-index: 0
机构:
Leibniz Lung Ctr, Res Ctr Borstel, Div Clin Infect Dis, Parkallee 35, DE-23845 Borstel, Germany
Clin TB Ctr, German Ctr Infect Res, Borstel, GermanyInst Salud Carlos III, Natl Ctr Microbiol, Mycol Reference Lab, Madrid, Spain
机构:
Univ Manchester, Univ S Manchester Hosp, Natl Aspergillosis Ctr, Manchester, Lancs, England
Manchester Acad Hlth Sci Ctr, Manchester, Lancs, EnglandUniv Manchester, Univ S Manchester Hosp, Natl Aspergillosis Ctr, Manchester, Lancs, England
Denning, David W.
;
Cadranel, Jacques
论文数: 0引用数: 0
h-index: 0
机构:
Hop Tenon, AP HP, Serv Pneumol, F-75970 Paris, France
Univ Paris 06, Sorbonne Univ, Paris, FranceUniv Manchester, Univ S Manchester Hosp, Natl Aspergillosis Ctr, Manchester, Lancs, England
Cadranel, Jacques
;
Beigelman-Aubry, Catherine
论文数: 0引用数: 0
h-index: 0
机构:
Univ Hosp CHUV, Dept Diagnost & Intervent Radiol, Lausanne, SwitzerlandUniv Manchester, Univ S Manchester Hosp, Natl Aspergillosis Ctr, Manchester, Lancs, England
Beigelman-Aubry, Catherine
;
Ader, Florence
论文数: 0引用数: 0
h-index: 0
机构:
Hosp Civils Lyon, Dept Infect Dis, Lyon, France
CNRS, INSERM, U1111, CIRI,UMR5308, Lyon, FranceUniv Manchester, Univ S Manchester Hosp, Natl Aspergillosis Ctr, Manchester, Lancs, England
Ader, Florence
;
Chakrabarti, Arunaloke
论文数: 0引用数: 0
h-index: 0
机构:
Postgrad Inst Med Educ & Res, Dept Med Microbiol, Ctr Adv Res Med Mycol, Chandigarh 160012, IndiaUniv Manchester, Univ S Manchester Hosp, Natl Aspergillosis Ctr, Manchester, Lancs, England
Chakrabarti, Arunaloke
;
Blot, Stijn
论文数: 0引用数: 0
h-index: 0
机构:
Univ Ghent, Dept Internal Med, B-9000 Ghent, Belgium
Univ Queensland, Burns Trauma & Crit Care Res Ctr, Brisbane, Qld, AustraliaUniv Manchester, Univ S Manchester Hosp, Natl Aspergillosis Ctr, Manchester, Lancs, England
Blot, Stijn
;
Ullmann, Andrew J.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Wurzburg, Dept Internal Med 2, Div Infect Dis, Univ Hosp Wurzburg, D-97070 Wurzburg, GermanyUniv Manchester, Univ S Manchester Hosp, Natl Aspergillosis Ctr, Manchester, Lancs, England
Ullmann, Andrew J.
;
Dimopoulos, George
论文数: 0引用数: 0
h-index: 0
机构:
Univ Athens, Sch Med, Univ Hosp Attikon, Dept Crit & Resp Care, GR-11527 Athens, GreeceUniv Manchester, Univ S Manchester Hosp, Natl Aspergillosis Ctr, Manchester, Lancs, England
机构:
Univ S Manchester Hosp, Manchester Acad Hlth Sci Ctr, Mycol Reference Ctr, Manchester M20 8LR, Lancs, EnglandUniv S Manchester Hosp, Manchester Acad Hlth Sci Ctr, Mycol Reference Ctr, Manchester M20 8LR, Lancs, England
Denning, David W.
;
Park, Steven
论文数: 0引用数: 0
h-index: 0
机构:
Univ Med & Dent New Jersey, New Jersey Med Sch, Publ Hlth Res Inst, Newark, NJ 07103 USAUniv S Manchester Hosp, Manchester Acad Hlth Sci Ctr, Mycol Reference Ctr, Manchester M20 8LR, Lancs, England
Park, Steven
;
Lass-Florl, Cornelia
论文数: 0引用数: 0
h-index: 0
机构:
Med Univ Innsbruck, Dept Hyg Mikrobiol & Sozalmed, Innsbruck, AustriaUniv S Manchester Hosp, Manchester Acad Hlth Sci Ctr, Mycol Reference Ctr, Manchester M20 8LR, Lancs, England
Lass-Florl, Cornelia
;
Fraczek, Marcin G.
论文数: 0引用数: 0
h-index: 0
机构:
Univ S Manchester Hosp, Manchester Acad Hlth Sci Ctr, Mycol Reference Ctr, Manchester M20 8LR, Lancs, EnglandUniv S Manchester Hosp, Manchester Acad Hlth Sci Ctr, Mycol Reference Ctr, Manchester M20 8LR, Lancs, England
Fraczek, Marcin G.
;
Kirwan, Marie
论文数: 0引用数: 0
h-index: 0
机构:Univ S Manchester Hosp, Manchester Acad Hlth Sci Ctr, Mycol Reference Ctr, Manchester M20 8LR, Lancs, England
Kirwan, Marie
;
Gore, Robin
论文数: 0引用数: 0
h-index: 0
机构:Univ S Manchester Hosp, Manchester Acad Hlth Sci Ctr, Mycol Reference Ctr, Manchester M20 8LR, Lancs, England
Gore, Robin
;
Smith, Jaclyn
论文数: 0引用数: 0
h-index: 0
机构:Univ S Manchester Hosp, Manchester Acad Hlth Sci Ctr, Mycol Reference Ctr, Manchester M20 8LR, Lancs, England
Smith, Jaclyn
;
Bueid, Ahmed
论文数: 0引用数: 0
h-index: 0
机构:Univ S Manchester Hosp, Manchester Acad Hlth Sci Ctr, Mycol Reference Ctr, Manchester M20 8LR, Lancs, England
Bueid, Ahmed
;
Moore, Caroline B.
论文数: 0引用数: 0
h-index: 0
机构:
Univ S Manchester Hosp, Manchester Acad Hlth Sci Ctr, Mycol Reference Ctr, Manchester M20 8LR, Lancs, EnglandUniv S Manchester Hosp, Manchester Acad Hlth Sci Ctr, Mycol Reference Ctr, Manchester M20 8LR, Lancs, England
Moore, Caroline B.
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Bowyer, Paul
论文数: 0引用数: 0
h-index: 0
机构:Univ S Manchester Hosp, Manchester Acad Hlth Sci Ctr, Mycol Reference Ctr, Manchester M20 8LR, Lancs, England
Bowyer, Paul
;
Perlin, David S.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Med & Dent New Jersey, New Jersey Med Sch, Publ Hlth Res Inst, Newark, NJ 07103 USAUniv S Manchester Hosp, Manchester Acad Hlth Sci Ctr, Mycol Reference Ctr, Manchester M20 8LR, Lancs, England
机构:
Univ Manchester, Manchester Acad Hlth Sci Ctr, Univ S Manchester Hosp, Natl Aspergillosis Ctr, Manchester M23 9LT, Lancs, EnglandUniv Manchester, Manchester Acad Hlth Sci Ctr, Univ S Manchester Hosp, Natl Aspergillosis Ctr, Manchester M23 9LT, Lancs, England
Kosmidis, Chris
;
Denning, David W.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Manchester, Manchester Acad Hlth Sci Ctr, Univ S Manchester Hosp, Natl Aspergillosis Ctr, Manchester M23 9LT, Lancs, EnglandUniv Manchester, Manchester Acad Hlth Sci Ctr, Univ S Manchester Hosp, Natl Aspergillosis Ctr, Manchester M23 9LT, Lancs, England
机构:
Inst Salud Carlos III, Natl Ctr Microbiol, Mycol Reference Lab, Madrid, SpainInst Salud Carlos III, Natl Ctr Microbiol, Mycol Reference Lab, Madrid, Spain
Alastruey-Izquierdo, Ana
;
Cadranel, Jacques
论文数: 0引用数: 0
h-index: 0
机构:
Hop Tenon, AP HP, Serv Pneumol, Paris, France
Sorbonne Univ, Paris, FranceInst Salud Carlos III, Natl Ctr Microbiol, Mycol Reference Lab, Madrid, Spain
Cadranel, Jacques
;
Flick, Holger
论文数: 0引用数: 0
h-index: 0
机构:
Med Univ Graz, Div Pulmonol, Dept Internal Med, Graz, AustriaInst Salud Carlos III, Natl Ctr Microbiol, Mycol Reference Lab, Madrid, Spain
机构:
UPMC Univ Paris 06, Sorbonne Univ, CRSA, INSERM, Paris, France
Hop St Antoine, AP HP, Serv Parasitol Mycol, Paris, FranceInst Salud Carlos III, Natl Ctr Microbiol, Mycol Reference Lab, Madrid, Spain
Hennequin, Christophe
;
论文数: 引用数:
h-index:
机构:
Hoenigl, Martin
;
Kosmidis, Chris
论文数: 0引用数: 0
h-index: 0
机构:
Manchester Univ Fdn Trust, Natl Aspergillosis Ctr, Manchester, Lancs, England
Univ Manchester, Div Infect Immun & Resp Med, Fac Biol Med & Hlth, Manchester Acad Hlth Sci Ctr, Manchester, Lancs, EnglandInst Salud Carlos III, Natl Ctr Microbiol, Mycol Reference Lab, Madrid, Spain
Kosmidis, Chris
;
论文数: 引用数:
h-index:
机构:
Lange, Christoph
;
Munteanu, Oxana
论文数: 0引用数: 0
h-index: 0
机构:
State Univ Med & Pharm Nicolae Testemitanu, Div Pneumol & Allergol, Dept Internal Med, Kishinev, MoldovaInst Salud Carlos III, Natl Ctr Microbiol, Mycol Reference Lab, Madrid, Spain
Munteanu, Oxana
;
Page, Iain
论文数: 0引用数: 0
h-index: 0
机构:
Clin TB Ctr, German Ctr Infect Res, Borstel, GermanyInst Salud Carlos III, Natl Ctr Microbiol, Mycol Reference Lab, Madrid, Spain
Page, Iain
;
Salzer, Helmut J. F.
论文数: 0引用数: 0
h-index: 0
机构:
Leibniz Lung Ctr, Res Ctr Borstel, Div Clin Infect Dis, Parkallee 35, DE-23845 Borstel, Germany
Clin TB Ctr, German Ctr Infect Res, Borstel, GermanyInst Salud Carlos III, Natl Ctr Microbiol, Mycol Reference Lab, Madrid, Spain
机构:
Univ Manchester, Univ S Manchester Hosp, Natl Aspergillosis Ctr, Manchester, Lancs, England
Manchester Acad Hlth Sci Ctr, Manchester, Lancs, EnglandUniv Manchester, Univ S Manchester Hosp, Natl Aspergillosis Ctr, Manchester, Lancs, England
Denning, David W.
;
Cadranel, Jacques
论文数: 0引用数: 0
h-index: 0
机构:
Hop Tenon, AP HP, Serv Pneumol, F-75970 Paris, France
Univ Paris 06, Sorbonne Univ, Paris, FranceUniv Manchester, Univ S Manchester Hosp, Natl Aspergillosis Ctr, Manchester, Lancs, England
Cadranel, Jacques
;
Beigelman-Aubry, Catherine
论文数: 0引用数: 0
h-index: 0
机构:
Univ Hosp CHUV, Dept Diagnost & Intervent Radiol, Lausanne, SwitzerlandUniv Manchester, Univ S Manchester Hosp, Natl Aspergillosis Ctr, Manchester, Lancs, England
Beigelman-Aubry, Catherine
;
Ader, Florence
论文数: 0引用数: 0
h-index: 0
机构:
Hosp Civils Lyon, Dept Infect Dis, Lyon, France
CNRS, INSERM, U1111, CIRI,UMR5308, Lyon, FranceUniv Manchester, Univ S Manchester Hosp, Natl Aspergillosis Ctr, Manchester, Lancs, England
Ader, Florence
;
Chakrabarti, Arunaloke
论文数: 0引用数: 0
h-index: 0
机构:
Postgrad Inst Med Educ & Res, Dept Med Microbiol, Ctr Adv Res Med Mycol, Chandigarh 160012, IndiaUniv Manchester, Univ S Manchester Hosp, Natl Aspergillosis Ctr, Manchester, Lancs, England
Chakrabarti, Arunaloke
;
Blot, Stijn
论文数: 0引用数: 0
h-index: 0
机构:
Univ Ghent, Dept Internal Med, B-9000 Ghent, Belgium
Univ Queensland, Burns Trauma & Crit Care Res Ctr, Brisbane, Qld, AustraliaUniv Manchester, Univ S Manchester Hosp, Natl Aspergillosis Ctr, Manchester, Lancs, England
Blot, Stijn
;
Ullmann, Andrew J.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Wurzburg, Dept Internal Med 2, Div Infect Dis, Univ Hosp Wurzburg, D-97070 Wurzburg, GermanyUniv Manchester, Univ S Manchester Hosp, Natl Aspergillosis Ctr, Manchester, Lancs, England
Ullmann, Andrew J.
;
Dimopoulos, George
论文数: 0引用数: 0
h-index: 0
机构:
Univ Athens, Sch Med, Univ Hosp Attikon, Dept Crit & Resp Care, GR-11527 Athens, GreeceUniv Manchester, Univ S Manchester Hosp, Natl Aspergillosis Ctr, Manchester, Lancs, England
机构:
Univ S Manchester Hosp, Manchester Acad Hlth Sci Ctr, Mycol Reference Ctr, Manchester M20 8LR, Lancs, EnglandUniv S Manchester Hosp, Manchester Acad Hlth Sci Ctr, Mycol Reference Ctr, Manchester M20 8LR, Lancs, England
Denning, David W.
;
Park, Steven
论文数: 0引用数: 0
h-index: 0
机构:
Univ Med & Dent New Jersey, New Jersey Med Sch, Publ Hlth Res Inst, Newark, NJ 07103 USAUniv S Manchester Hosp, Manchester Acad Hlth Sci Ctr, Mycol Reference Ctr, Manchester M20 8LR, Lancs, England
Park, Steven
;
Lass-Florl, Cornelia
论文数: 0引用数: 0
h-index: 0
机构:
Med Univ Innsbruck, Dept Hyg Mikrobiol & Sozalmed, Innsbruck, AustriaUniv S Manchester Hosp, Manchester Acad Hlth Sci Ctr, Mycol Reference Ctr, Manchester M20 8LR, Lancs, England
Lass-Florl, Cornelia
;
Fraczek, Marcin G.
论文数: 0引用数: 0
h-index: 0
机构:
Univ S Manchester Hosp, Manchester Acad Hlth Sci Ctr, Mycol Reference Ctr, Manchester M20 8LR, Lancs, EnglandUniv S Manchester Hosp, Manchester Acad Hlth Sci Ctr, Mycol Reference Ctr, Manchester M20 8LR, Lancs, England
Fraczek, Marcin G.
;
Kirwan, Marie
论文数: 0引用数: 0
h-index: 0
机构:Univ S Manchester Hosp, Manchester Acad Hlth Sci Ctr, Mycol Reference Ctr, Manchester M20 8LR, Lancs, England
Kirwan, Marie
;
Gore, Robin
论文数: 0引用数: 0
h-index: 0
机构:Univ S Manchester Hosp, Manchester Acad Hlth Sci Ctr, Mycol Reference Ctr, Manchester M20 8LR, Lancs, England
Gore, Robin
;
Smith, Jaclyn
论文数: 0引用数: 0
h-index: 0
机构:Univ S Manchester Hosp, Manchester Acad Hlth Sci Ctr, Mycol Reference Ctr, Manchester M20 8LR, Lancs, England
Smith, Jaclyn
;
Bueid, Ahmed
论文数: 0引用数: 0
h-index: 0
机构:Univ S Manchester Hosp, Manchester Acad Hlth Sci Ctr, Mycol Reference Ctr, Manchester M20 8LR, Lancs, England
Bueid, Ahmed
;
Moore, Caroline B.
论文数: 0引用数: 0
h-index: 0
机构:
Univ S Manchester Hosp, Manchester Acad Hlth Sci Ctr, Mycol Reference Ctr, Manchester M20 8LR, Lancs, EnglandUniv S Manchester Hosp, Manchester Acad Hlth Sci Ctr, Mycol Reference Ctr, Manchester M20 8LR, Lancs, England
Moore, Caroline B.
;
Bowyer, Paul
论文数: 0引用数: 0
h-index: 0
机构:Univ S Manchester Hosp, Manchester Acad Hlth Sci Ctr, Mycol Reference Ctr, Manchester M20 8LR, Lancs, England
Bowyer, Paul
;
Perlin, David S.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Med & Dent New Jersey, New Jersey Med Sch, Publ Hlth Res Inst, Newark, NJ 07103 USAUniv S Manchester Hosp, Manchester Acad Hlth Sci Ctr, Mycol Reference Ctr, Manchester M20 8LR, Lancs, England
机构:
Univ Manchester, Manchester Acad Hlth Sci Ctr, Univ S Manchester Hosp, Natl Aspergillosis Ctr, Manchester M23 9LT, Lancs, EnglandUniv Manchester, Manchester Acad Hlth Sci Ctr, Univ S Manchester Hosp, Natl Aspergillosis Ctr, Manchester M23 9LT, Lancs, England
Kosmidis, Chris
;
Denning, David W.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Manchester, Manchester Acad Hlth Sci Ctr, Univ S Manchester Hosp, Natl Aspergillosis Ctr, Manchester M23 9LT, Lancs, EnglandUniv Manchester, Manchester Acad Hlth Sci Ctr, Univ S Manchester Hosp, Natl Aspergillosis Ctr, Manchester M23 9LT, Lancs, England