Epithelial Na+ channel mutants causing Liddle's syndrome retain ability to respond to aldosterone and vasopressin

被引:46
作者
Auberson, M
Hoffman-Pochon, N
Vandewalle, A
Kellenberger, S
Schild, L
机构
[1] Univ Lausanne, Inst Pharmacol & Toxicol, CH-1005 Lausanne, Switzerland
[2] INSERM, U478, Fac Med Xavier Bichat, F-75870 Paris 18, France
关键词
collecting duct; PY motif; hypertension;
D O I
10.1152/ajprenal.00071.2003
中图分类号
Q4 [生理学];
学科分类号
071003 ;
摘要
Liddle's syndrome is a monogenic form of hypertension caused by mutations in the PY motif of the COOH terminus of beta- and gamma-epithelial Na+ channel ( ENaC) subunits. These mutations lead to retention of active channels at the cell surface. Because of the critical role of this PY motif in the stability of ENaCs at the cell surface, we have investigated its contribution to the ENaC response to aldosterone and vasopressin. Mutants of the PY motif in beta- and gamma-ENaC subunits (beta-Y618A, beta-P616L, beta-R564stop, and gamma-K570stop) were stably expressed by retroviral gene transfer in a renal cortical collecting duct cell line (mpkCCD(cl4)), and transepithelial Na+ transport was assessed by measurements of the benzamil-sensitive short-circuit current (I-sc). Cells that express ENaC mutants of the PY motif showed a five- to sixfold higher basal I-sc compared with control cells and responded to stimulation by aldosterone (10(-6) M) or vasopressin (10(-9) M) with a further increase in I-sc. The rates of the initial increases in I-sc after aldosterone or vasopressin stimulation were comparable in cells transduced with wild-type and mutant ENaCs, but reversal of the effects of aldosterone and vasopressin was slower in cells that expressed the ENaC mutants. The conserved sensitivity of ENaC mutants to stimulation by aldosterone and vasopressin together with the prolonged activity at the cell surface likely contribute to the increased Na+ absorption in the distal nephron of patients with Liddle's syndrome.
引用
收藏
页码:F459 / F471
页数:13
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