AtsPLA2-α nuclear relocalization by the Arabidopsis transcription factor AtMYB30 leads to repression of the plant defense response

被引:77
作者
Froidure, Solene [1 ]
Canonne, Joanne [1 ]
Daniel, Xavier [1 ]
Jauneau, Alain [2 ]
Briere, Christian [3 ]
Roby, Dominique [1 ]
Rivas, Susana [1 ]
机构
[1] Ctr Natl Rech Sci, Unite Mixte Rech, LIPM, Inst Natl Rech Agronom 2594 441, F-31320 Castanet Tolosan, France
[2] Inst Federatif Rech 40, Pole Biotechnol Vegetale, F-31320 Castanet Tolosan, France
[3] Univ Toulouse 3, Ctr Natl Rech Sci, Univ Toulouse Unite Mixte Rech, F-31326 Castanet Tolosan, France
关键词
hypersensitive response; MYB transcription factor; secretory phospholipase; plant disease resistance; HYPERSENSITIVE CELL-DEATH; CYTOSOLIC PHOSPHOLIPASE A(2); CYSTEINE PROTEASE; INNATE IMMUNITY; R2R3-MYB GENE; ACTIVATION; THALIANA; ACID; IDENTIFICATION; RESISTANCE;
D O I
10.1073/pnas.1009056107
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
The hypersensitive response (HR), characterized by a rapid and localized cell death at the inoculation site, is one of the most efficient resistance reactions to pathogen attack in plants. The transcription factor AtMYB30 was identified as a positive regulator of the HR and resistance responses during interactions between Arabidopsis and bacteria. Here, we show that AtMYB30 and the secreted phospholipase AtsPLA(2)-alpha physically interact in vivo, following the AtMYB30-mediated specific relocalization of AtsPLA(2)-alpha from cytoplasmic vesicles to the plant cell nucleus. This protein interaction leads to repression of AtMYB30 transcriptional activity and negative regulation of plant HR. Moreover, Atspla(2)-alpha mutant plants are more resistant to bacterial inoculation, whereas AtsPLA(2)-alpha overexpression leads to decreased resistance, confirming that AtsPLA(2)-alpha is a negative regulator of AtMYB30-mediated defense. These data underline the importance of cellular dynamics and, particularly, protein translocation to the nucleus, for defense-associated gene regulation in plants.
引用
收藏
页码:15281 / 15286
页数:6
相关论文
共 41 条
[1]   RD19, an Arabidopsis cysteine protease required for RRS1-R-mediated resistance, is relocalized to the nucleus by the Ralstonia solanacearum PopP2 effector [J].
Bernoux, Maud ;
Timmers, Ton ;
Jauneau, Alain ;
Briere, Christian ;
de Wit, Pierre J. G. M. ;
Marco, Yves ;
Deslandes, Laurent .
PLANT CELL, 2008, 20 (08) :2252-2264
[2]   Impact of phyto-oxylipins in plant defense [J].
Blée, E .
TRENDS IN PLANT SCIENCE, 2002, 7 (07) :315-321
[3]   Biology of Secretory Phospholipase A2 [J].
Boyanovsky, Boris B. ;
Webb, Nancy R. .
CARDIOVASCULAR DRUGS AND THERAPY, 2009, 23 (01) :61-72
[4]   Chloroplastic protein NRIP1 mediates innate immune receptor recognition of a viral effector [J].
Caplan, Jeffrey L. ;
Mamillapalli, Padmavathi ;
Burch-Smith, Tessa M. ;
Czymmek, Kirk ;
Dinesh-Kumar, S. P. .
CELL, 2008, 132 (03) :449-462
[5]   Activation of phospholipase a by plant defense elicitors [J].
Chandra, S ;
Heinstein, PF ;
Low, PS .
PLANT PHYSIOLOGY, 1996, 110 (03) :979-986
[6]   A novel myb oncogene homologue in Arabidopsis thaliana related to hypersensitive cell death [J].
Daniel, X ;
Lacomme, C ;
Morel, JB ;
Roby, D .
PLANT JOURNAL, 1999, 20 (01) :57-66
[7]   NPR1, all things considered [J].
Dong, XN .
CURRENT OPINION IN PLANT BIOLOGY, 2004, 7 (05) :547-552
[8]   Regulation of the Arabidopsis defense transcriptome [J].
Eulgem, T .
TRENDS IN PLANT SCIENCE, 2005, 10 (02) :71-78
[9]   Arabidopsis SENESCENCE-ASSOCIATED GENE101 stabilizes and signals within an ENHANCED DISEASE SUSCEPTIBILITY1 complex in plant innate immunity [J].
Feys, BJ ;
Wiermer, M ;
Bhat, RA ;
Moisan, LJ ;
Medina-Escobar, N ;
Neu, C ;
Cabral, A ;
Parker, JE .
PLANT CELL, 2005, 17 (09) :2601-2613
[10]   Oxylipin profiling reveals the preferential stimulation of the 9-lipoxygenase pathway in elicitor-treated potato cells [J].
Göbel, C ;
Feussner, I ;
Schmidt, A ;
Scheel, D ;
Sanchez-Serrano, J ;
Hamberg, M ;
Rosahl, S .
JOURNAL OF BIOLOGICAL CHEMISTRY, 2001, 276 (09) :6267-6273