Protein identification using 2D-LC-MS/MS

被引：143

作者：

Delahunty, C ^{[1
]}

Yates, JR ^{[1
]}

机构：

[1] Scripps Res Inst, Dept Cell Biol, La Jolla, CA 92037 USA

来源：

METHODS | 2005年 / 35卷 / 03期

关键词：

proteomics; multidimensional chromatography; mass spectrometry; protein mixture analysis;

D O I：

10.1016/j.ymeth.2004.08.016

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Multidimensional liquid chromatography techniques have been coupled to tandem mass spectrometry to provide a robust method to identify proteins in complex mixtures. Data acquisition is interfaced directly with search algorithms for identification through cross-correlation with databases. This review describes the most recent advances in methodologies for protein identification by mass spectrometry and describes the limitations of the application of the technologies. (c) 2004 Elsevier Inc. All rights reserved.

引用

页码：248 / 255

页数：8

共 31 条

[1] Phospho-regulation of kinetochore-microtubule attachments by the aurora kinase Ipl1p [J].

Cheeseman, LM ;

Anderson, S ;

Jwa, M ;

Green, EM ;

Kang, JS ;

Yates, JR ;

Chan, CSM ;

Drubin, DG ;

Barnes, G .

CELL, 2002, 111 (02) :163-172

[2] A MICROSCALE ELECTROSPRAY INTERFACE FOR ONLINE, CAPILLARY LIQUID-CHROMATOGRAPHY TANDEM MASS-SPECTROMETRY OF COMPLEX PEPTIDE MIXTURES [J].

DAVIS, MT ;

STAHL, DC ;

HEFTA, SA ;

LEE, TD .

ANALYTICAL CHEMISTRY, 1995, 67 (24) :4549-4556

[3] Cln3 activates G1-specific transcription via phosphorylation of the SBF transcription bound repressor Whi5 [J].

de Bruin, RAM ;

McDonald, WH ;

Kalashnikova, TI ;

Yates, J ;

Wittenberg, C .

CELL, 2004, 117 (07) :887-898

[4] MICRO-ELECTROSPRAY MASS-SPECTROMETRY - ULTRA-HIGH-SENSITIVITY ANALYSIS OF PEPTIDES AND PROTEINS [J].

EMMETT, MR ;

CAPRIOLI, RM .

JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY, 1994, 5 (07) :605-613

[5] AN APPROACH TO CORRELATE TANDEM MASS-SPECTRAL DATA OF PEPTIDES WITH AMINO-ACID-SEQUENCES IN A PROTEIN DATABASE [J].

ENG, JK ;

MCCORMACK, AL ;

YATES, JR .

JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY, 1994, 5 (11) :976-989

[6] A proteomic view of the Plasmodium falciparum life cycle [J].

Florens, L ;

Washburn, MP ;

Raine, JD ;

Anthony, RM ;

Grainger, M ;

Haynes, JD ;

Moch, JK ;

Muster, N ;

Sacci, JB ;

Tabb, DL ;

Witney, AA ;

Wolters, D ;

Wu, YM ;

Gardner, MJ ;

Holder, AA ;

Sinden, RE ;

Yates, JR ;

Carucci, DJ .

NATURE, 2002, 419 (6906) :520-526

[7] Protein identification at the low femtomole level from silver-stained gels using a new fritless electrospray interface for liquid chromatography microspray and nanospray mass spectrometry [J].

Gatlin, CL ;

Kleemann, GR ;

Hays, LG ;

Link, AJ ;

Yates, JR .

ANALYTICAL BIOCHEMISTRY, 1998, 263 (01) :93-101

[8] Direct database searching with MALDI-PSD spectra of peptides [J].

Griffin, PR ;

MacCoss, MJ ;

Eng, JK ;

Blevins, RA ;

Aaronson, JS ;

Yates, JR .

RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 1995, 9 (15) :1546-1551

[9] STRUCTURAL-ANALYSIS OF PROTEINS BY CAPILLARY HPLC ELECTROSPRAY TANDEM MASS-SPECTROMETRY [J].

GRIFFIN, PR ;

COFFMAN, JA ;

HOOD, LE ;

YATES, JR .

INTERNATIONAL JOURNAL OF MASS SPECTROMETRY AND ION PROCESSES, 1991, 111 :131-149

[10] Quantitative analysis of complex protein mixtures using isotope-coded affinity tags [J].

Gygi, SP ;

Rist, B ;

Gerber, SA ;

Turecek, F ;

Gelb, MH ;

Aebersold, R .

NATURE BIOTECHNOLOGY, 1999, 17 (10) :994-999

← 1 2 3 4 →