Comparison of RT-qPCR results of different gene targets for SARS-CoV-2 in asymptomatic individuals during COVID-19 pandemic

被引：0

作者：

Tuncel, Gulten ^{[1
]}

Ergoren, Mahmut Cerkez ^{[1
,2
]}

Baddal, Buket ^{[1
,3
]}

Tulay, Pinar ^{[1
,2
]}

Arikan, Ayse ^{[1
,3
]}

Guler, Emrah ^{[1
,4
]}

Ozverel, Cenk Serhan ^{[1
,5
]}

Suer, H. Kaya ^{[6
]}

Sayan, Murat ^{[1
,7
]}

Sanlidag, Tamer ^{[1
]}

机构：

[1] Near East Univ, DESAM Inst, Nicosia, Cyprus

[2] Near East Univ, Dept Med Genet, Fac Med, Nicosia, Cyprus

[3] Near East Univ, Fac Med, Dept Clin Microbiol & Med Microbiol, Nicosia, Cyprus

[4] Near East Univ, Fac Hlth Sci, Nicosia, Cyprus

[5] Near East Univ, Dept Basic Med Sci, Fac Dent, Nicosia, Cyprus

[6] Near East Univ, Fac Med, Dept Infect Dis & Clin Microbiol, Nicosia, Cyprus

[7] Kocaeli Univ, Educ & Res Hosp, PCR Unit, Kocaeli, Turkey

[8] Near East Univ, DESAM Inst, COVID 19 PCR Lab, CY-99138 Nicosia, Cyprus

来源：

EUROBIOTECH JOURNAL | 2021年 / 5卷

关键词：

SARS-CoV-2; COVID-19; RT-qPCR; asymptomatic; nucleocapsid; RdRp; CORONAVIRUS OUTBREAK; HEALTH;

D O I：

10.2478/ebtj-2021-0018

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

A reverse transcriptase-quantitative polymerase chain reaction (RT-qPCR) is regarded as the most sensitive method available and is being used for screening procedure for all incoming passengers to Northern Cyprus for SARS-CoV-2. This study investigated the compatibility of two different RT-qPCR methodologies Diagnovital (R) and Bio-Speedy (R) by re-analyzing the previously confirmed positive samples. A total of 43 previously confirmed positive samples were re-analyzed by two different commercially available SARS-CoV-2 RT-qPCR kits. Only 23.5% of positive samples detected by Diagnovitar RTqPCR kit were detected by Bio-Speedy (R) detection kit. In conclusion, adoption of Diagnovital (R) RT-qPCR kit detecting two regions of SARS-CoV-2 genome in our laboratories enabled the detection of SARS-CoV-2 in asymptomatic cases with higher sensitivity and contributed to the prevention of viral transmission within the country. The timely detection of infection in asymptomatic individuals may be the key to a successful fight against the COVID-19 pandemic.

引用

页码：26 / 31

页数：6

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