Effect of S/N ratio on sulfide removal by autotrophic denitrification

被引：89

作者：

Dolejs, Petr ^{[1
]}

Paclik, Ladislav ^{[1
]}

Maca, Josef ^{[1
]}

Pokorna, Dana ^{[1
]}

Zabranska, Jana ^{[1
]}

Bartacek, Jan ^{[1
]}

机构：

[1] Inst Chem Technol, Fac Environm Technol, Dept Water Technol & Environm Engn, CR-16628 Prague 6, Czech Republic

来源：

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY | 2015年 / 99卷 / 05期

关键词：

Autotrophic denitrification; Completely stirred tank reactor (CSTR); S/N ratio; Sulfide removal; Thiobacillus denitrificans; Thiomicrospira denitrificans; HYDROGEN-SULFIDE; THIOBACILLUS-DENITRIFICANS; SULFUR-COMPOUNDS; NITRATE REMOVAL; WASTE-WATER; OXIDATION; REACTORS; SYSTEM; KINETICS; CULTURE;

D O I：

10.1007/s00253-014-6140-6

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

In this study a completely stirred tank reactor was used to study the effect of sulfide to nitrate (S/N) ratio on sulfide removal while nitrate was used as electron acceptor. Several S/N ratios were studied for this purpose ranging from 0.3 to 2.4 mol/mol. The complete sulfide removal was achieved when S/N ratio 0.85 mol/mol was used with the autotrophic denitrification efficiency up to 80 %. No nitrite accumulation was observed, and the main product of sulfide oxidation was sulfate. Dissimilatory nitrogen reduction to ammonia occurred and subsequently, elemental sulfur accumulated while S/N ratio was higher than 1.3 mol/mol. The specific autotrophic denitrification rates under S/N ratios 0.8 and 1.2 were 5 and 26 mg g(-1) h(-1) (N-NO3 (-), VSS), respectively. Thiobacillus denitrificans and Thiomicrospira denitrificans were detected in the reactor by fluorescent in situ hybridization, but their overall representation was not more than 5 % of the entire microbial populations.

引用

页码：2383 / 2392

页数：10

共 37 条

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