Inula viscosa (L.) Aiton leaves and flower buds: Effect of extraction solvent/technique on their antioxidant ability, antimicrobial properties and phenolic profile

被引：30

作者：

Mohti, H. ^{[1
,2
]}

Taviano, M. F. ^{[3
]}

Cacciola, F. ^{[4
]}

Dugo, P. ^{[3
,5
,6
]}

Mondello, L. ^{[3
,5
,6
]}

Marino, A. ^{[3
]}

Crisafi, G. ^{[3
]}

Benameur, Q. ^{[7
]}

Zaid, A. ^{[1
,2
]}

Miceli, N. ^{[3
]}

机构：

[1] Moulay Ismail Univ, Fac Sci, Dept Biol, Meknes, Morocco

[2] CUI UMI UHasselt Program, Morocco, Belgium

[3] Univ Messina, Polo Annunziata, Dipartimento Sci Chim Biol Farmaceut & Ambientali, Messina, Italy

[4] Univ Messina, Dipartimento Sci Biomed Odontoiatr & Immagini Mor, Messina, Italy

[5] Univ Campus Biomed Rome, Fac Dipartimentale Med & Chirurg, Rome, Italy

[6] Univ Messina, Dipartimento Sci Chim Biol Farmaceut & Ambientali, Chromaleont Srl, Messina, Italy

[7] Abdelhamid Ibn Badis Univ, Fac Nat Sci & Life, Nursing Dept, Mostaganem, Algeria

来源：

NATURAL PRODUCT RESEARCH | 2020年 / 34卷 / 01期

关键词：

Inula viscosa (L; ) Aiton; extraction solvent; technique; antioxidant activity; antimicrobial activity; Artemia salina Leach; phenolic compounds; HPLC-PDA-ESI-MS; PLANTS;

D O I：

10.1080/14786419.2019.1569659

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

This study was designed to establish the most effective solvent/technique for extracting antioxidant phytoconstituents from leaves and flower buds of Inula viscosa (L.) Aiton (Asteraceae) grown wild in Morocco. Maceration and hot extraction with methanol or water and Soxhlet ethanol extraction were utilized. The antioxidant potential was evaluated in vitro by DPPH, reducing power, and ferrous ions chelating activity assays. I. viscosa leaf and flower bud extracts displayed the strongest effect in the DPPH test, being the Soxhlet ethanol the most active ones (IC50 = 54.24 +/- 0.21 mu g/mL and 39.77 +/- 0.23 mu g/mL); thus, they were selected for further investigations. The antimicrobial efficacy of the Soxhlet ethanol extracts against ATCC and food isolates strains was assayed; the leaf extract showed the best activity, and Candida albicans was the most sensitive strain (MIC = 125 mu g/mL). The extracts resulted non-toxic against Artemia salina. Among the phenolics characterised by HPLC-PDA-ESI-MS, hispidulin hexoside, patuletin and spinacetin were identified for the first time.

引用

页码：46 / 52

页数：7

共 18 条

[1] Antibacterial activity of Cladanthus arabicus and Bubonium imbricatum essential oils alone and in combination with conventional antibiotics against Enterobacteriaceae isolates [J].