A novel simple biosensor containing silver nanoparticles/propolis (bee glue) for microRNA let-7a determination

被引：43

作者：

Elhakim, Heba K. A. ^{[1
]}

Azab, Shereen M. ^{[2
]}

Fekry, Amany M. ^{[3
]}

机构：

[1] Cairo Univ, Biochem Dept, Fac Sci, Giza 12613, Egypt

[2] Natl Org Drug Control & Res NODCAR, Pharmaceut Chem Dept, 6 Abu Hazem St,Pyramids Ave,POB 29, Giza, Egypt

[3] Cairo Univ, Fac Sci, Phys Chem Dept, Giza 12613, Egypt

来源：

MATERIALS SCIENCE AND ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS | 2018年 / 92卷

关键词：

MicroRNAs; Ag nanoparticles; Propolis; TEM; EIS; ELECTROCHEMICAL BIOSENSOR; GOLD NANOPARTICLES; SENSOR; ELECTROPHORESIS; HYBRIDIZATION;

D O I：

10.1016/j.msec.2018.06.063

中图分类号：

TB3 [工程材料学]; R318.08 [生物材料学];

学科分类号：

0805 ; 080501 ; 080502 ;

摘要：

A novel sensitive electrochemical sensor for microRNAlet-7a detection in normal serum samples, hepatocellular carcinoma patients and human liver cancer cells, has been excellently synthesized. The sensor constructed of carbon paste (CP) amended with silver nanoparticles (AgNPs) and extracted propolis (bee glue). The AgNPs/P modified carbon paste electrode (APCPE) displayed a high electrocatalytic activity in a Britton Robinson (BR) buffer (pH = 7.4). The techniques utilized to prepare this work are square wave voltammetry (SWV) and electrochemical impedance spectroscopy (EIS). Surface characteristics were achieved using scanning (SEM), Fourier-transform infrared spectroscopy (FTTR), Spectrophotometer, transmission (TEM) electron microscope, energy dispersive X-ray analysis (EDX) and elemental mapping (EM) techniques. Under optimal conditions, the suggested sensor exhibits good rapid and sensible response reaching a very low detection limit of 10(-3) femtomolar.

引用

页码：489 / 495

页数：7

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