Protoplast isolation and plant regeneration of different doubled haploid lines of cauliflower (Brassica oleracea var. botrytis)

被引：19

作者：

Sheng, Xiaoguang ^{[1
]}

Zhao, Zhenqing ^{[1
]}

Yu, Huifang ^{[1
]}

Wang, Jiansheng ^{[1
]}

Zhang Xiaohui ^{[1
]}

Gu, Honghui ^{[1
]}

机构：

[1] Zhejiang Acad Agr Sci, Inst Vegetables, Hangzhou 310021, Zhejiang, Peoples R China

来源：

PLANT CELL TISSUE AND ORGAN CULTURE | 2011年 / 107卷 / 03期

关键词：

Cauliflower; Protoplast; Plant regeneration; Ploidy level; SRAP analysis; INTERGENERIC SOMATIC HYBRIDS; RICE PROTOPLASTS; CULTURE; SYSTEM; CALLI; SRAP;

D O I：

10.1007/s11240-011-0002-z

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Protoplasts were isolated from hypocotyls of 5-day-old seedlings of five doubled-haploid (DH) lines of cauliflower after enzymatic digestion in 0.1% macerozyme R-10 and 1.0% cellulose R-10. Shoot regeneration was achieved in four of the five DH lines. Protoplast yield and frequency of cell division and shoot regeneration varied among experiments and DH lines. Sequence-related amplified polymorphism (SRAP) analysis on all the regenerated plants of each DH line indicated that their genetic compositions were homologous with their mother plants, but few regenerated plants of DH lines no. 3 and 5 were detected with changes in ploidy levels.

引用

页码：513 / 520

页数：8

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