Cell type-specific gene expression profiling in brain tissue: comparison between TRAP, LCM and RNA-seq

被引：14
作者：
Kim, TaeHyun [1 ]
Lim, Chae-Seok [1 ]
Kaang, Bong-Kiun [1 ]
机构：
[1] Seoul Natl Univ, Dept Biol Sci, Seoul 151747, South Korea
来源：
BMB REPORTS | 2015年 / 48卷 / 07期
基金：
新加坡国家研究基金会;
关键词：
Brain; Cell type-specific; Gene expression profiling; Neuron; Transcriptome; LASER-CAPTURE MICRODISSECTION; NEURONS; TRANSCRIPTOME; DISEASE; RETINA; LTP;
D O I：
10.5483/BMBRep.2015.48.7.218
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The brain is an organ that consists of various cell types. As our knowledge of the structure and function of the brain progresses, cell type-specific research is gaining importance. Together with advances in sequencing technology and bioinformatics, cell type-specific transcriptome studies are providing important insights into brain cell function. In this review, we discuss 3 different cell type-specific transcriptome analyses i.e., Laser Capture Microdissection (LCM), Translating Ribosome Affinity Purification (TRAP)/RiboTag, and single cell RNA-Seq, that are widely used in the field of neuroscience.
引用
收藏
页码：388 / 394
页数：7
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