Cell type-specific gene expression profiling in brain tissue: comparison between TRAP, LCM and RNA-seq

被引：14

作者：

Kim, TaeHyun ^{[1
]}

Lim, Chae-Seok ^{[1
]}

Kaang, Bong-Kiun ^{[1
]}

机构：

[1] Seoul Natl Univ, Dept Biol Sci, Seoul 151747, South Korea

来源：

BMB REPORTS | 2015年 / 48卷 / 07期

基金：

新加坡国家研究基金会;

关键词：

Brain; Cell type-specific; Gene expression profiling; Neuron; Transcriptome; LASER-CAPTURE MICRODISSECTION; NEURONS; TRANSCRIPTOME; DISEASE; RETINA; LTP;

D O I：

10.5483/BMBRep.2015.48.7.218

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The brain is an organ that consists of various cell types. As our knowledge of the structure and function of the brain progresses, cell type-specific research is gaining importance. Together with advances in sequencing technology and bioinformatics, cell type-specific transcriptome studies are providing important insights into brain cell function. In this review, we discuss 3 different cell type-specific transcriptome analyses i.e., Laser Capture Microdissection (LCM), Translating Ribosome Affinity Purification (TRAP)/RiboTag, and single cell RNA-Seq, that are widely used in the field of neuroscience.

引用

页码：388 / 394

页数：7

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