S100A9 Interaction with TLR4 Promotes Tumor Growth

被引：113

作者：

Kallberg, Eva ^{[1
]}

Vogl, Thomas ^{[2
]}

Liberg, David ^{[3
]}

Olsson, Anders ^{[3
]}

Bjork, Per ^{[3
]}

Wikstrom, Pernilla ^{[4
]}

Bergh, Anders ^{[4
]}

Roth, Johannes ^{[2
]}

Ivars, Fredrik ^{[1
]}

Leanderson, Tomas ^{[1
]}

机构：

[1] Lund Univ, Immunol Grp, Lund, Sweden

[2] Univ Munster, Inst Immunol, Munster, Germany

[3] Act Biotech AB, Lund, Sweden

[4] Umea Univ, Dept Med Biosci, Umea, Sweden

来源：

PLOS ONE | 2012年 / 7卷 / 03期

关键词：

RESISTANT PROSTATE-CANCER; ANTI-ANGIOGENIC AGENT; SUPPRESSOR-CELLS; TGF-BETA; TRANSGENIC ADENOCARCINOMA; AUTOIMMUNE-DISEASE; IN-VIVO; PROTEINS; MOUSE; IDENTIFICATION;

D O I：

10.1371/journal.pone.0034207

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

By breeding TRAMP mice with S100A9 knock-out (S100A9(-/-)) animals and scoring the appearance of palpable tumors we observed a delayed tumor growth in animals devoid of S100A9 expression. CD11b(+) S100A9 expressing cells were not observed in normal prostate tissue from control C57BL/6 mice but were readily detected in TRAMP prostate tumors. Also, S100A9 expression was observed in association with CD68(+) macrophages in biopsies from human prostate tumors. Delayed growth of TRAMP tumors was also observed in mice lacking the S100A9 ligand TLR4. In the EL-4 lymphoma model tumor growth inhibition was observed in S100A9(-/-) and TLR4(-/-), but not in RAGE(-/-) animals lacking an alternative S100A9 receptor. When expression of immune-regulating genes was analyzed using RT-PCR the only common change observed in mice lacking S100A9 and TLR4 was a down-regulation of TGF beta expression in splenic CD11b(+) cells. Lastly, treatment of mice with a small molecule (ABR-215050) that inhibits S100A9 binding to TLR4 inhibited EL4 tumor growth. Thus, S100A9 and TLR4 appear to be involved in promoting tumor growth in two different tumor models and pharmacological inhibition of S100A9-TLR4 interactions is a novel and promising target for anti-tumor therapies.

引用

页数：11

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[21] Tumour-mediated upregulation of chemoattractants and recruitment of myeloid cells predetermines lung metastasis [J].