Evaluation antithrombotic activity and action mechanism of myricitrin

Myricitrin was isolated from Cercis chinensis leaves and its antithrombotic activity was evaluated by activated partial thromboplastin time (APTT), thrombin time (TT), prothrombin time (PT), and fibrinogen (FIB) in vitro and rat acute blood stasis model in vivo. APTT, PT and TT were significantly prolonged and plasma FIB was significantly shortened in myricitrin group compared with model group in vitro. In rat acute blood stasis model, myricitrin (5 mg/mL and 2.5 mg/mL) could extend APTT, PT and TT, increase the level of 6-keto prostaglandin F-1 alpha (6-keto-PGF1 alpha) and nitric oxide synthase (eNOS), decrease the level of plasma FIB, thromboxane B-2 (TXB2), endothelin-1 (ET-1), blood sedimentation (ESR), hematocrit (PCV), whole blood viscosity (WBV) and plasma viscosity (PV). All of above revealed that myricitrin had good antithrombotic effects.