Assessment of metabolic fluxes in the mouse brain in vivo using 1H-[13C] NMR spectroscopy at 14.1 Tesla

被引:19
作者
Xin, Lijing [1 ,2 ]
Lanz, Bernard [1 ]
Lei, Hongxia [3 ,4 ]
Gruetter, Rolf [1 ,3 ,5 ]
机构
[1] Ecole Polytech Fed Lausanne, Lab Funct & Metab Imaging LIFMET, CH-1015 Lausanne, Switzerland
[2] Lausanne Univ Hosp CHUV, Ctr Psychiat Neurosci, Dept Psychiat, Unit Res Schizophrenia, Lausanne, Switzerland
[3] Univ Geneva, Dept Radiol, Geneva, Switzerland
[4] Ecole Polytech Fed Lausanne, Anim Imaging & Technol Core AIT, Ctr Biomed Imaging CIBM, CH-1015 Lausanne, Switzerland
[5] Univ Lausanne, Dept Radiol, Lausanne, Switzerland
基金
瑞士国家科学基金会;
关键词
energy metabolism; glucose; glutamate; lactate; MR spectroscopy; RAT-BRAIN; TCA CYCLE; H-1-NMR SPECTROSCOPY; GLUCOSE-METABOLISM; NEUROCHEMICAL PROFILE; C-13; ACETATE; TIME; QUANTIFICATION; TRANSPORT;
D O I
10.1038/jcbfm.2014.251
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
C-13 magnetic resonance spectroscopy (MRS) combined with the administration of C-13 labeled substrates uniquely allows to measure metabolic fluxes in vivo in the brain of humans and rats. The extension to mouse models may provide exclusive prospect for the investigation of models of human diseases. In the present study, the short-echo-time (TE) full-sensitivity H-1-[C-13] MRS sequence combined with high magnetic field (14.1 T) and infusion of [U-C-13(6)] glucose was used to enhance the experimental sensitivity in vivo in the mouse brain and the C-13 turnover curves of glutamate C4, glutamine C4, glutamate+glutamine C3, aspartate C2, lactate C3, alanine C3, gamma-aminobutyric acid C2, C3 and C4 were obtained. A one-compartment model was used to fit C-13 turnover curves and resulted in values of metabolic fluxes including the tricarboxylic acid (TCA) cycle flux V-TCA (1.05 +/- 0.04 mu mol/g per minute), the exchange flux between 2-oxoglutarate and glutamate V-x (0.48 +/- 0.02 mu mol/g per minute), the glutamate-glutamine exchange rate V-gln (0.20 +/- 0.02 mu mol/g per minute), the pyruvate dilution factor K-dil (0.82 +/- 0.01), and the ratio for the lactate conversion rate and the alanine conversion rate V-Lac/V-Ala (10 +/- 2). This study opens the prospect of studying transgenic mouse models of brain pathologies.
引用
收藏
页码:759 / 765
页数:7
相关论文
共 43 条
  • [31] Pfeuffer J, 1999, MAGNET RESON MED, V41, P1077, DOI 10.1002/(SICI)1522-2594(199906)41:6<1077::AID-MRM1>3.0.CO
  • [32] 2-#
  • [33] ESTIMATION OF METABOLITE CONCENTRATIONS FROM LOCALIZED IN-VIVO PROTON NMR-SPECTRA
    PROVENCHER, SW
    [J]. MAGNETIC RESONANCE IN MEDICINE, 1993, 30 (06) : 672 - 679
  • [34] Astrocyte-mediated control of cerebral blood flow
    Takano, T
    Tian, GF
    Peng, WG
    Lou, NH
    Libionka, W
    Han, XN
    Nedergaard, M
    [J]. NATURE NEUROSCIENCE, 2006, 9 (02) : 260 - 267
  • [35] Glutamatergic and GABAergic TCA cycle and neurotransmitter cycling fluxes in different regions of mouse brain
    Tiwari, Vivek
    Ambadipudi, Susmitha
    Patel, Anant B.
    [J]. JOURNAL OF CEREBRAL BLOOD FLOW AND METABOLISM, 2013, 33 (10) : 1523 - 1531
  • [36] Highly resolved in vivo 1H NMR spectroscopy of the mouse brain at 9.4 T
    Tkác, I
    Henry, PG
    Andersen, P
    Keene, CD
    Low, WC
    Gruetter, R
    [J]. MAGNETIC RESONANCE IN MEDICINE, 2004, 52 (03) : 478 - 484
  • [37] Developmental and regional changes in the neurochemical profile of the rat brain determined by in vivo 1H NMR spectroscopy
    Tkác, I
    Rao, R
    Georgieff, MK
    Gruetter, R
    [J]. MAGNETIC RESONANCE IN MEDICINE, 2003, 50 (01) : 24 - 32
  • [38] Tkác I, 1999, MAGNET RESON MED, V41, P649, DOI 10.1002/(SICI)1522-2594(199904)41:4<649::AID-MRM2>3.0.CO
  • [39] 2-G
  • [40] Mathematical modeling of 13C label incorporation of the TCA cycle:: The concept of composite precursor function
    Uffmann, Kai
    Gruetter, Rolf
    [J]. JOURNAL OF NEUROSCIENCE RESEARCH, 2007, 85 (15) : 3304 - 3317