Bacterial populations in juvenile maize rhizospheres originate from both seed and soil

被引:183
作者
Johnston-Monje, David [1 ,2 ]
Lundberg, Derek S. [3 ]
Lazarovits, George [4 ]
Reis, Veronica M. [5 ]
Raizada, Manish N. [1 ]
机构
[1] Univ Guelph, Dept Plant Agr, Guelph, ON N1G 2W1, Canada
[2] Symbiota, 100 Edwin H Land Blvd, Cambridge, MA 02142 USA
[3] Max Planck Inst Dev Biol, Spemannstr 35, D-72076 Tubingen, Germany
[4] Agroecol Res Serv Ctr, A&L Biol, 2136 Jetstream Rd, London, ON N5V 3P5, Canada
[5] Embrapa Agrobiol, Km 47,Estr Antiga Rio Sao Paulo, BR-23890000 Rio De Janeiro, Brazil
关键词
Endophyte; Epiphyte; Zeamays; Maize; Bacteria; 16S; Microbial ecology; Root; Rhizosphere; Rhizoplane; Endosphere; Phyllosphere; Spermosphere; Seed; TRFLP; Miseq; Next-generation sequencing; Soil; Terra preta; Vertical transmission; Colonization; Inoculation; Microbiome; ENDOPHYTIC BACTERIA; MICROBIAL COMMUNITY; RICE SEEDS; DIVERSITY; ROOTS; GROWTH; COLONIZATION; AZOSPIRILLUM; GENOTYPE; ANCIENT;
D O I
10.1007/s11104-016-2826-0
中图分类号
S3 [农学(农艺学)];
学科分类号
0901 ;
摘要
To assess the impacts of soil microbes and plant genotype on the composition of maize associated bacterial communities. Two genotypes of Brazilian maize were planted indoors on sterile sand, a deep underground subsoil, and a nutrient-rich topsoil from the Amazon jungle (terra preta). DNA was extracted from rhizospheres, phyllospheres, and surface sterilized roots for 16S rDNA fingerprinting and next generation sequencing. Neither plant genotype nor soil type appeared to influence bacterial diversity in phyllospheres or endospheres. Rhizospheres showed strikingly similar 16S rDNA ordination of both fingerprinting and sequencing data, with soil type driving grouping patterns and genotype having a significant impact only on sterile sand. Rhizospheres grown in non-sterile soils contained greater bacterial diversity than sterile-sand grown ones, however the dominant OTUs (species of Proteobacteria and Bacteroidetes) were found in all rhizospheres suggesting seeds as a common source of inoculum. Rhizospheres of the commercial hybrid appeared to contain less bacterial diversity than the landrace. Maize rhizospheres receive diverse bacteria from soil, are influenced by the genotype or treatment of the seed, and are dominated by species of Proteobacteria, Actinobacteria, Bacteroidetes, and Firmicutes. As many dominant 16S rDNA sequences were observed in rhizospheres grown in both sterile and non-sterile substrate, we conclude that the most common bacterial cells in juvenile maize rhizospheres are seed transmitted.
引用
收藏
页码:337 / 355
页数:19
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