Oxidative stress induced pyroptosis leads to osteogenic dysfunction of MG63 cells

被引：73

作者：

Liu, Shanshan ^{[1
,2
,3
]}

Du, Juan ^{[1
,2
,3
,4
]}

Li, Dongfang ^{[1
,2
,3
]}

Yang, Panpan ^{[1
,2
,3
]}

Kou, Yuying ^{[1
,2
,3
]}

Li, Congshan ^{[1
,2
,3
]}

Zhou, Qin ^{[1
,2
,3
]}

Lu, Yupu ^{[1
,2
,3
]}

Hasegawa, Tomoka ^{[5
]}

Li, Minqi ^{[1
,2
,3
]}

机构：

[1] Shandong Univ, Sch & Hosp Stomatol, Dept Bone Metab, Jinan 250012, Peoples R China

[2] Shandong Key Lab Oral Tissue Regenerat, Jinan 250012, Peoples R China

[3] Shandong Engn Lab Dent Mat & Oral Tissue Regenera, Jinan 250012, Peoples R China

[4] Shandong First Med Univ, Dept Oral & Maxillofacial Surg, Shandong Prov Hosp, Jinan 250021, Peoples R China

[5] Hokkaido Univ, Grad Sch Dent Med, Dept Dev Biol Hard Tissue, Sapporo, Hokkaido 0608586, Japan

来源：

JOURNAL OF MOLECULAR HISTOLOGY | 2020年 / 51卷 / 03期

关键词：

Periodontitis; Osteoblast; Oxidative stress; Pyroptosis; Migration; Mineralization; NLRP3 INFLAMMASOME ACTIVATION; OSTEOBLAST DIFFERENTIATION; BONE-RESORPTION; FREE-RADICALS; APOPTOSIS; LIPOPOLYSACCHARIDE; PERIODONTITIS; EXPRESSION; TISSUE; PATHOGENESIS;

D O I：

10.1007/s10735-020-09874-9

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Periodontitis is characterized by alveolar bone destruction and is one of the most common chronic oral diseases. Inflammatory cytokines released by pyroptosis, which can be triggered by oxidative stress, are critical in the development of periodontitis. This study aims to clarify whether oxidative stress causes osteoblast dysfunction by inducing pyroptosis in the process of periodontitis. We found that treatment with lipopolysaccharide (LPS) led to NLRP3 inflammasome-mediated pyroptosis of MG63 cells as well as decreased cell migration. Of note, LPS stimulation increased LDH release in a time- and dose-dependent manner. However, inhibition of reactive oxygen species with N-acetyl-L-cysteine attenuated oxidative stress-mediated pyroptosis and improved migration injury in osteoblasts treated with LPS. Further, inhibition of the NLRP3 inflammasome with MCC950 improved osteoblast migration and restored the expression of osteogenic differentiation-related proteins such as COL 1, RUNX 2 and ALP. In conclusion, oxidative stress caused by LPS induces pyroptosis in osteoblasts, leading to osteogenic dysfunction.

引用

页码：221 / 232

页数：12

共 46 条

[1] Repression of TXNIP-NLRP3 axis restores intestinal barrier function via inhibition of myeloperoxidase activity and oxidative stress in nonalcoholic steatohepatitis [J].