Ultrasensitive electrochemiluminescence immunosensor with wide linear range based on a multiple amplification approach

被引：15

作者：

Huo, Xiao-Lei ^{[1
,2
]}

Zhang, Nan ^{[1
,2
]}

Xu, Jing-Juan ^{[1
,2
]}

Chen, Hong-Yuan ^{[1
,2
]}

机构：

[1] Nanjing Univ, Sch Chem & Chem Engn, State Key Lab Analyt Chem Life Sci, Nanjing 210023, Jiangsu, Peoples R China

[2] Nanjing Univ, Sch Chem & Chem Engn, Collaborat Innovat Ctr Chem Life Sci, Nanjing 210023, Jiangsu, Peoples R China

来源：

ELECTROCHEMISTRY COMMUNICATIONS | 2019年 / 98卷

基金：

中国国家自然科学基金;

关键词：

Ultrasensitive; Electrochemiluminescence; Multiple amplification; PGA; PSA; PROSTATE-SPECIFIC ANTIGEN; PEPTIDE-BASED BIOSENSOR; CANCER BIOMARKER; LUMINOL-ELECTROCHEMILUMINESCENCE; SIGNAL AMPLIFICATION; SENSITIVE DETECTION; QUANTUM DOTS; GRAPHENE; NANOPARTICLES; NANOMATERIALS;

D O I：

10.1016/j.elecom.2018.11.014

中图分类号：

O646 [电化学、电解、磁化学];

学科分类号：

081704 ;

摘要：

An ultrasensitive and highly selective electrochemiluminescence (ECL) immunosensor is proposed for the detection of prostate specific antigen (PSA) based on multiple amplification via glucose oxidase (GOx) and platinum nanoparticles (Pt NPs) conjugated with polyglutamic acid (PGA). The proposed immunosensor has a wide linear range (1.0 pg/mL-150 ng/mL) and low detection limit (0.6 pg/mL) for PSA detection, which is due in part to the excellent catalytic activity of the Ab(2)-GOx-PGA-Pt NPs probe toward the luminol ECL reaction.

引用

页码：33 / 37

页数：5

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