A solid supported reagent for internucleoside H-phosphonate linkage formation

被引：1
作者：
Mohe, N
Heinonen, P
Sanghvi, YS
Strömberg, R
机构：
[1] Karolinska Inst, Scheele Lab, Div Organ & Bioorgan Chem, MBB, S-17177 Stockholm, Sweden
[2] Isis Pharmaceut Inc, Carlsbad, CA USA
来源：
NUCLEOSIDES NUCLEOTIDES & NUCLEIC ACIDS | 2005年 / 24卷 / 5-7期
关键词：
D O I：
10.1081/NCN-200059257
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A fast and convenient procedure for synthesis of dinucleoside H-phosphonates is obtained through use of the novel polystyrene supported 5-carboxy-5-methyl-2-oxo- 2-chloro-1,3,2-dioxaphosphorinane reagent. Virtually quantitative H-phosphonate condensations are obtained leading to excellent isolated yields and with only a simple filtration as the purification procedure. This provides for a convenient and high-yielding procedure that should be suited for solution-phase synthesis of oligonucleotides.
引用
收藏
页码：897 / 899
页数：3
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