Dual role of oxidative stress-JNK activation in autophagy and apoptosis induced by nickel oxide nanoparticles in human cancer cells

被引:31
作者
Cho, Yik-Lam [1 ,2 ]
Tan, Hayden Weng Siong [1 ,3 ]
Saquib, Quaiser [4 ]
Ren, Yi [1 ,2 ]
Ahmad, Javed [4 ]
Wahab, Rizwan [4 ]
He, Weifeng [5 ]
Bay, Boon-Huat [2 ]
Shen, Han-Ming [1 ,3 ,6 ]
机构
[1] Natl Univ Singapore, Yong Loo Lin Sch Med, Dept Physiol, Singapore, Singapore
[2] Natl Univ Singapore, Yong Loo Lin Sch Med, Dept Anat, Singapore, Singapore
[3] Natl Univ Singapore, Grad Sch Integrat Sci & Engn, Singapore, Singapore
[4] King Saud Univ, Zool Dept, Coll Sci, Riyadh, Saudi Arabia
[5] Army Med Univ, Southwest Hosp, Inst Burn Res, State Key Lab Trauma Burn & Combined Injury, Chongqing, Peoples R China
[6] Univ Macau, Fac Hlth Sci, Macau, Peoples R China
基金
英国医学研究理事会;
关键词
Apoptosis; Autophagy; NiO-NPs; Oxidative stress; JNK; ROS; MESENCHYMAL STEM-CELLS; NIO NANOPARTICLES; REACTIVE OXYGEN; CYTOTOXICITY; PROTEIN; LC3; ENHANCEMENT; SURVIVAL; PATHWAY; DAMAGE;
D O I
10.1016/j.freeradbiomed.2020.03.027
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Nickel oxide nanoparticles (NiO-NPs) are an important group of nanoparticles with increasing applications in many aspects of industry. At present, there is evidence demonstrating the cytotoxic characteristics of NiO-NPs, while the involvement of autophagy in the cytotoxicity of NiO-NPs has not been reported. In this study, we aimed to study the role of autophagy in the cytotoxicity of NiO-NPs and the underlying regulatory mechanisms. First, we provided evidence that NiO-NPs induce autophagy in human cancer cells. Second, we found that the enhanced autophagic flux by NiO-NPs via the generation of intracellular reactive oxygen species (ROS) from mitochondria and the subsequent activation of the JNK pathway. Third, we demonstrated that the activation of JNK is a main force in mediating NiO-NPs-induced apoptosis. Finally, we demonstrated that the autophagic response plays an important protective role against the cytotoxic effect of NiO-NPs. Therefore, this study identifies the dual role of oxidative stress-JNK activation in the biological effects of NiO-NPs via promoting autophagy and mediating apoptosis. Understanding the protective role of autophagy and the underlying mechanism is important for the potential application of NiO-NPs in the biomedical industry.
引用
收藏
页码:173 / 186
页数:14
相关论文
共 82 条
[1]   Nickel oxide nanoparticles are highly toxic to SH-SY5Y neuronal cells [J].
Abudayyak, Mahmoud ;
Guzel, Elif ;
Ozhan, Gul .
NEUROCHEMISTRY INTERNATIONAL, 2017, 108 :7-14
[2]   Cytotoxicity and apoptotic effects of nickel oxide nanoparticles in cultured HeLa cells [J].
Ada, Kezban ;
Turk, Mustafa ;
Oguztuzun, Serpil ;
Kilic, Murat ;
Demirel, Mehmet ;
Tandogan, Nisa ;
Ersayar, Ertan ;
Latif, Ozturk .
FOLIA HISTOCHEMICA ET CYTOBIOLOGICA, 2010, 48 (04) :524-529
[3]   Role of Reactive Oxygen Species in Cancer Progression: Molecular Mechanisms and Recent Advancements [J].
Aggarwal, Vaishali ;
Tuli, Hardeep Singh ;
Varol, Aysegul ;
Thakral, Falak ;
Yerer, Mukerrem Betul ;
Sak, Katrin ;
Varol, Mehmet ;
Jain, Aklank ;
Khan, Asaduzzaman ;
Sethi, Gautam .
BIOMOLECULES, 2019, 9 (11)
[4]   Nickel oxide nanoparticles exert cytotoxicity via oxidative stress and induce apoptotic response in human liver cells (HepG2) [J].
Ahamed, Maqusood ;
Ali, Daoud ;
Alhadlaq, Hisham A. ;
Akhtar, Mohd Javed .
CHEMOSPHERE, 2013, 93 (10) :2514-2522
[5]  
[Anonymous], INT J MOL SCI
[6]  
[Anonymous], MOL MECH UNDERLYING
[7]  
[Anonymous], ENV GEOCHEM HLTH
[8]  
[Anonymous], CURR MED CHEM
[9]  
[Anonymous], HUMAN AGS GASTRIC CA
[10]  
[Anonymous], ROLE AUTOPHAGY NANOP