Inhibition of cyclooxygenase-2 sensitizes lung cancer cells to radiation-induced apoptosis

被引:13
作者
Han, Zhi-Qiang [1 ]
Liao, Hongwei [2 ]
Shi, Feng [1 ]
Chen, Xiao-Ping [1 ]
Hu, Hua-Cheng [3 ]
Tian, Ming-Qing [1 ]
Wang, Li-Hua [1 ]
Ying, Songmin [2 ]
机构
[1] Peoples Hosp Quzhou City, Dept Resp Internal Med, 2 Zhongdilou Rd, Quzhou 324000, Zhejiang, Peoples R China
[2] Zhejiang Univ, Sch Med, Inst Resp Dis, 866 Yuhangtang Rd, Hangzhou 310058, Zhejiang, Peoples R China
[3] Suzhou Univ, Affiliated Hosp 2, Dept Resp Internal Med, Suzhou 215004, Jiangsu, Peoples R China
关键词
cyclooxygenase-2; lung cancer; synergist; synthetic lethal; PHASE-III TRIAL; OVARIAN-CANCER; COX-2; PROTEIN; EXPRESSION; INDUCTION; CHEMOTHERAPY; MANAGEMENT; DISCOVERY; RADIOSENSITIVITY; RADIOTHERAPY;
D O I
10.3892/ol.2017.6940
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
Radiotherapy resistance is an enduring major setback in lung cancer therapy, and is responsible for a large proportion of treatment failures. In previous years, cyclooxygenase-2 (COX-2) has frequently been reported to promote tumor occurrence and development, suggesting a potential role in radiotherapy resistance. To investigate whether COX-2 inhibitors can be applied in radiosensitization, an MTT assay was performed to examine cell viability after X-ray radiation in the presence or absence of the specific COX-2 inhibitor Celecoxib. Cell apoptosis and cell cycle changes were also detected through laser confocal scanning microcopy and flow cytometry. X-ray treatment only caused mild cell death in lung cancer A549 cells. However, combination treatment using celecoxib and X-ray radiation exhibited improved inhibitory effects and significantly suppressed cell proliferation. Therefore, COX-2 inhibitors combined with radiotherapy can counteract radiation-induced high COX-2 expression, demonstrating that celecoxib can function as a radiosensitizer of lung cancer cells. It is therefore reasonable to predict COX-2 inhibitors to be potential clinical radiotherapy synergists.
引用
收藏
页码:5959 / 5965
页数:7
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