A simple method to design PCR primer to detect genomic DNA of parasites and its application to Dirofilaria immitis

被引：5

作者：

Nagano, I

Wu, ZL

Nakayama, M

Takahashi, Y

机构：

[1] GIFU UNIV,SCH MED,DEPT PARASITOL,GIFU 500,JAPAN

[2] NAKAYAMA VET HOSP,NARA 630,JAPAN

来源：

MOLECULAR AND CELLULAR PROBES | 1996年 / 10卷 / 06期

关键词：

PCR; primer; Dirofilaria immitis;

D O I：

10.1006/mcpr.1996.0058

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A simple method to design a polymerase chain reaction (PCR) primer for parasites of interest was developed using Dirofilaria immitis as a test sample. The method involved the cloning and sequencing of randomly amplified DNA of the parasite, and designing a primer based on the resulting DNA sequence. Using the primer, DNA fragments of the expected length were amplified by a regular PCR with genomic DNA of Dirofilaria immitis. (C) 1996 Academic Press Limited

引用

页码：423 / 425

页数：3

共 7 条

[1] DIROFILARIA REPENS - CLONING AND CHARACTERIZATION OF A REPEATED DNA-SEQUENCE FOR THE DIAGNOSIS OF DIROFILARIASIS IN DOGS, CANIS-FAMILIARIS [J].