Estimation of low frequency antigen-presenting cells with a novel RELISPOT assay

被引：2

作者：

Dzutsev, Amiran K. ^{[1
]}

Belyakov, Igor A. ^{[1
]}

Isakov, Dmitry V. ^{[1
]}

Gagnon, Susan J. ^{[1
]}

Margulies, David H. ^{[2
]}

Berzofsky, Jay A. ^{[1
]}

机构：

[1] NCI, Ctr Canc Res, Vaccine Branch, NIH, Bethesda, MD 20892 USA

[2] NIAID, NIH, Immunol Lab, Bethesda, MD 20892 USA

来源：

JOURNAL OF IMMUNOLOGICAL METHODS | 2008年 / 333卷 / 1-2期

关键词：

antigen-presenting cell; T-cell line; ELISPOT; RELISPOT; dendritic cell; migration; vaccinia virus;

D O I：

10.1016/j.jim.2008.01.008

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Adequate presentation of self and foreign antigens is a key factor for efficient T-cell immunosurveillance against pathogens and tumors. Cells presenting foreign antigens usually comprise a rare population and are difficult to detect even at the peak of infection. Here we demonstrate a CD8(+) T-cell-based approach that allows detection of specific antigen-presenting cells (APC) at a frequency of less than 0.0005%. When T cells are in excess, they form rosettes with rare APCs, which appear as single spots in an IFN-gamma ELISPOT assay. Using this RELISPOT (Rosette ELISPOT) method we demonstrate the dynamic interplay between CD8 T cells and professional and non-professional APCs following virus challenge. Published by Elsevier B.V.

引用

页码：71 / 78

页数：8

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