Numerical method for optimization of thermal cycling in 3D-printed polymerase chain reaction device

被引：3

作者：

Park, Jaehyun ^{[1
]}

Park, Heesung ^{[1
]}

机构：

[1] Changwon Natl Univ, Dept Mech Engn, 20 Changwondaehak Ro, Chang Won 51140, South Korea

来源：

MICROELECTRONIC ENGINEERING | 2020年 / 227卷

基金：

新加坡国家研究基金会;

关键词：

3D-printer; Microchannel; Polymerase chain reaction; Thermal cycling; DNA AMPLIFICATION; CONTINUOUS-FLOW; HEAT-TRANSFER; PCR; MICROCHANNELS;

D O I：

10.1016/j.mee.2020.111306

中图分类号：

TM [电工技术]; TN [电子技术、通信技术];

学科分类号：

0808 ; 0809 ;

摘要：

A continuous-flow polymerase chain reaction (PCR) device with integrated heaters for DNA amplification was studied. We developed a numerical model to predict the temperature variations of a 3D-printed PCR device for different heater temperatures. The target temperatures at thermal zones for a successful PCR are 95 degrees C, 55 degrees C, and 72 degrees C for denaturation, annealing and extension, respectively. A numerical simulation was conducted to determine the heater temperatures required to compensate for heat loss. Our numerical model and optimization method were confirmed by comparison with the experimental results. Consequently, the compensated heater temperatures required to achieve the target temperatures are 97.9 degrees C, 56.6 degrees C and 74.1 degrees C. The proposed optimization method and numerical simulations will be useful in achieving highly effective thermal cycling in the 3D-printed PCR device.

引用

页数：7

共 21 条

[1] Incorporating asymmetric PCR and microarray hybridization protocols onto an integrated microfluidic device, screening for the Escherichia coli ssrA gene [J].

Brennan, Des ;

Glynn, Barry ;

Keegan, Gemma ;

McDonagh, Colette ;

Barry, Thomas ;

Galvin, Paul .

SENSORS AND ACTUATORS B-CHEMICAL, 2018, 261 :325-334

[2] Nucleic acid amplification using microfluidic systems [J].

Chang, Chen-Min ;

Chang, Wen-Hsin ;

Wang, Chih-Hung ;

Wang, Jung-Hao ;

Mai, John D. ;

Lee, Gwo-Bin .

LAB ON A CHIP, 2013, 13 (07) :1225-1242

[3] Small but Perfectly Formed? Successes, Challenges, and Opportunities for Microfluidics in the Chemical and Biological Sciences [J].

Chiu, Daniel T. ;

deMello, Andrew J. ;

Di Carlo, Dino ;

Doyle, Patrick S. ;

Hansen, Carl ;

Maceiczyk, Richard M. ;

Wootton, Robert C. R. .

CHEM, 2017, 2 (02) :201-223

[4] Extreme PCR: Efficient and Specific DNA Amplification in 15-60 Seconds [J].

Farrar, Jared S. ;

Wittwer, Carl T. .

CLINICAL CHEMISTRY, 2015, 61 (01) :145-153

[5] Integration of isothermal amplification methods in microfluidic devices: Recent advances [J].

Giuffrida, Maria Chiara ;

Spoto, Giuseppe .

BIOSENSORS & BIOELECTRONICS, 2017, 90 :174-186

[6]

Hardt S, 2004, NSTI NANOTECH 2004, VOL 1, TECHNICAL PROCEEDINGS, P55

[7] Three-Dimensional Transient Thermal Analysis for a Silicon PCR Microreactor [J].

Ibrahim, Omar ;

Jones, Benjamin ;

Hassab, Mohammed ;

Op de Beeck, Maaike .

NUMERICAL HEAT TRANSFER PART A-APPLICATIONS, 2014, 65 (11) :1069-1088

[8] Ultrafast, low-power, PCB manufacturable, continuous-flow microdevice for DNA amplification [J].

Kaprou, Georgia D. ;

Papadopoulos, Vasileios ;

Papageorgiou, Dimitris P. ;

Kefala, Ioanna ;

Papadakis, George ;

Gizeli, Electra ;

Chatzandroulis, Stavros ;

Kokkoris, George ;

Tserepi, Angeliki .

ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 2019, 411 (20) :5297-5307

[9] Chemical amplification: Continuous-flow PCR on a chip [J].

Kopp, MU ;

de Mello, AJ ;

Manz, A .

SCIENCE, 1998, 280 (5366) :1046-1048

[10] Bubble-free rapid microfluidic PCR [J].

Lee, Sang Hun ;

Song, Jihwan ;

Cho, Byungrae ;

Hong, SoonGweon ;

Hoxha, Ori ;

Kang, Taewook ;

Kim, Dongchoul ;

Lee, Luke P. .

BIOSENSORS & BIOELECTRONICS, 2019, 126 :725-733

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