Effects of encapsulation on the viability of probiotic strains exposed to lethal conditions

被引:13
作者
Borges, Sandra [1 ]
Barbosa, Joana [1 ]
Camilo, Rute [1 ]
Carvalheira, Ana [1 ]
Silva, Joana [1 ]
Sousa, Sergio [1 ]
Gomes, Ana M. [1 ]
Pintado, Maria M. [1 ]
Silva, Jose P. [2 ]
Costa, Paulo [2 ]
Amaral, Maria H. [2 ]
Teixeira, Paula [1 ]
Freitas, Ana C. [3 ,4 ,5 ]
机构
[1] CBQF Escola Super Biotecnol, P-4200072 Oporto, Portugal
[2] Univ Porto, Fac Farm, P-4050123 Oporto, Portugal
[3] Inst Piaget, ISEIT Viseu, P-3515776 Lordosa, Viseu, Portugal
[4] Univ Aveiro, CESAM, P-3810193 Aveiro, Portugal
[5] Univ Aveiro, Dept Chem, P-3810193 Aveiro, Portugal
关键词
Alginate; extrusion; lethal conditions; microencapsulation; probiotic bacteria; BIFIDOBACTERIUM-LACTIS; MICROENCAPSULATION; ALGINATE; SURVIVAL; LACTOBACILLUS; BACTERIA; MICROCAPSULES; STORAGE; DELIVERY; STARCH;
D O I
10.1111/j.1365-2621.2011.02855.x
中图分类号
TS2 [食品工业];
学科分类号
0832 ;
摘要
The effect of microencapsulation on the viability of Lactobacillus casei, L. paracasei, L. acidophilus Ki and Bifidobacterium animalis BB-12 during exposure to lethal conditions (25% NaCl, pH 3.0 and 55-60 degrees C) was evaluated. Results demonstrated that survival of probiotic strains to the imposed lethal stress conditions was strain dependent. With the exception of exposure to 25% (w/v) NaCl, L. acidophilus Ki (free and encapsulated cells) demonstrated the highest survival rates through exposure to lethal conditions of temperature and pH. For this probiotic strain exposed to heat, microencapsulated cells expressed a higher heat tolerance at 55 degrees C than free cells. For the other tested bacteria, in general, encapsulation had no positive effect on survival through the tested lethal conditions.
引用
收藏
页码:416 / 421
页数:6
相关论文
共 35 条
[1]   Microstructural studies of probiotic bacteria-loaded alginate microcapsules using standard electron microscopy techniques and anhydrous fixation [J].
Allan-Wojtas, P. ;
Hansen, L. Truelstrup ;
Paulson, A. T. .
LWT-FOOD SCIENCE AND TECHNOLOGY, 2008, 41 (01) :101-108
[2]   Effect of process variables on particle size and viability of Bifidobacterium lactis Bb-12 in genipin-gelatin microspheres [J].
Annan, N. T. ;
Borza, A. ;
Moreau, D. L. ;
Allan-Wojtas, P. M. ;
Truelstrup Hansen, L. .
JOURNAL OF MICROENCAPSULATION, 2007, 24 (02) :152-162
[3]   Aerodynamically assisted jetting and threading for processing concentrated suspensions containing advanced structural, functional and biological materials [J].
Arumuganathar, Sumathy ;
Suter, Nicolai ;
Walzel, Peter ;
Jayasinghe, Suwan N. .
Biotechnology Journal, 2009, 4 (01) :64-72
[4]   Effect of homogenisation on bead size and survival of encapsulated probiotic bacteria [J].
Capela, P. ;
Hay, T. K. C. ;
Shah, N. P. .
FOOD RESEARCH INTERNATIONAL, 2007, 40 (10) :1261-1269
[5]   Microencapsulation for the improved delivery of bioactive compounds into foods [J].
Champagne, Claude P. ;
Fustier, Patrick .
CURRENT OPINION IN BIOTECHNOLOGY, 2007, 18 (02) :184-190
[6]   GROWTH OF BIFIDOBACTERIA IN MILK AND PREPARATION OF BIFIDOBACTERIUM-INFANTIS FOR A DIETARY ADJUNCT [J].
COLLINS, EB ;
HALL, BJ .
JOURNAL OF DAIRY SCIENCE, 1984, 67 (07) :1376-1380
[7]   Synbiotic microcapsules that enhance microbial viability during nonrefrigerated storage and gastrointestinal transit [J].
Crittenden, R ;
Weerakkody, R ;
Sanguansri, L ;
Augustin, M .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2006, 72 (03) :2280-2282
[8]   Acid, bile, and heat tolerance of free and microencapsulated probiotic bacteria [J].
Ding, W. K. ;
Shah, N. P. .
JOURNAL OF FOOD SCIENCE, 2007, 72 (09) :M446-M450
[9]   Efficacy of whey protein gel networks as potential viability-enhancing scaffolds for cell immobilization of Lactobacillus rhamnosus GG [J].
Doherty, S. B. ;
Gee, V. L. ;
Ross, R. P. ;
Stanton, C. ;
Fitzgerald, G. F. ;
Brodkorb, A. .
JOURNAL OF MICROBIOLOGICAL METHODS, 2010, 80 (03) :231-241
[10]   Technologies with free and immobilised cells for probiotic bifidobacteria production and protection [J].
Doleyres, Y ;
Lacroix, C .
INTERNATIONAL DAIRY JOURNAL, 2005, 15 (10) :973-988