Application of oblique plane microscopy to high speed live cell imaging

被引：1

作者：

Kumar, Sunil ^{[1
]}

Wilding, Dean ^{[1
]}

Sikkel, Markus B. ^{[2
]}

Lyon, Alexander R. ^{[2
]}

MacLeod, Ken T. ^{[2
]}

Dunsby, Chris ^{[1
,3
]}

机构：

[1] Univ London Imperial Coll Sci Technol & Med, Dept Phys, Photon Grp, S Kensington Campus, London SW7 2AZ, England

[2] Natl Heart & Lung Inst, Imperial Coll London, London SW3 6LY, England

[3] Hammersmith Hosp, Imperial Coll LOndon, Dept Med, London W12 0HS, England

来源：

ADVANCED MICROSCOPY TECHNIQUES II | 2011年 / 8086卷

基金：

英国工程与自然科学研究理事会;

关键词：

Fluorescence microscopy; Three-dimensional microscopy; Selective Plane Illumination Microscopy; ILLUMINATION MICROSCOPY;

D O I：

10.1117/12.889841

中图分类号：

TH742 [显微镜];

学科分类号：

摘要：

Oblique Plane Microscopy (OPM) is a light sheet microscopy technique that combines oblique illumination with correction optics that tilt the focal plane of the collection system. OPM can be used to image conventionally mounted specimens on coverslips or tissue culture dishes and has low out-of-plane photobleaching and phototoxicity. No moving parts are required to achieve an optically sectioned image and so high speed optically sectioned imaging is possible. We present high speed 2D and 3D optically sectioned OPM imaging of live cells using a high NA water immersion lens.

引用

页数：5

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