Spectral Study on the Interaction of [Cu(DPPZ) (L-Ser)]+ Complex with DNA and Its Analytical Application

被引：0

作者：

Wu Cheng-ke ^{[1
]}

Dong Qiu-peng ^{[1
,2
]}

Zhang Hong ^{[1
]}

Xiong Cen ^{[1
]}

Wu Xiu-li ^{[1
]}

Ling Lian-sheng ^{[1
]}

机构：

[1] Sun Yat Sen Univ, Sch Chem & Chem Engn, Guangzhou 510275, Guangdong, Peoples R China

[2] Chinese Acad Sci, Guangzhou Inst Geochem, Guangzhou 510640, Peoples R China

来源：

SPECTROSCOPY AND SPECTRAL ANALYSIS | 2011年 / 31卷 / 04期

关键词：

Resonance light scattering; Copper; L-serine; Dipyrido[3,2-a; 2; 3; '-c]phenazine; Calf thymus DNA; RESONANCE LIGHT-SCATTERING; GOLD NANOPARTICLES; HYBRIDIZATION; SYSTEM; SIGNALS; PROBE; ASSAY;

D O I：

10.3964/j.issn.1000-0593(2011)04-1057-04

中图分类号：

O433 [光谱学];

学科分类号：

0703 ; 070302 ;

摘要：

The characteristics of resonance light scattering (RLS), UV-visible absorption spectra and fluorescence spectra of [Cu(DPPZ)(L-Ser)](+) with DNA were studied and a RLS method for the determination of DNA was established. [Cu(DPPZ)(L-Ser)](+) could assemble on the surface of DNA through intercalation, and enhanced the RLS of DNA in the tris buffer of pH 7. 2. The maximum resonance light scattering peak appeared at 400 nm. Under the optimum conditions, the enhanced intensity of RLS was proportional to the concentration of DNA over the range of 0. 42 similar to 4. 2 ng . mL(-1) , with a detection limit (3 sigma/k) of 0. 29 ng . mL(-1). The method was used for the determination of DNA samples with the recoveries between 97. 8% and 106%.

引用

页码：1057 / 1060

页数：4

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