Detection of Nesopora caninum-Specific DNA from Cerebrospinal Fluid by Polymerase Chain Reaction in a Dog with Confirmed Neosporosis

被引：9

作者：

Ishigaki, Kyohei ^{[2
]}

Noya, Masahiko ^{[2
]}

Kagawa, Yumiko ^{[3
]}

Ike, Kazunori ^{[1
]}

Orima, Hiromitsu ^{[4
]}

Imai, Soichi ^{[1
]}

机构：

[1] Nippon Vet & Life Sci Univ, Fac Vet Sci, Dept Vet Med, Lab Vet Parasitol, Musashino, Tokyo 1808602, Japan

[2] Noya Anim Hosp, Saitama 3501234, Japan

[3] North Lab, Shiroishi Ku, Sapporo, Hokkaido 0030027, Japan

[4] Nippon Vet & Life Sci Univ, Fac Vet Sci, Dept Vet Med, Lab Vet Radiol, Musashino, Tokyo 1808602, Japan

来源：

JOURNAL OF VETERINARY MEDICAL SCIENCE | 2012年 / 74卷 / 08期

关键词：

antemortem diagnosis; canine; Neospora caninum; neosporosis; PCR; SEROLOGICAL SURVEY; CLINICAL SIGNS; DIAGNOSIS; INFECTION; TACHYZOITES; PROTOZOAN; JAPAN; MICE; PCR;

D O I：

10.1292/jvms.11-0341

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

A one-month male Greyhound dog presented with a swinging gait of the hindlimbs, and later developed muscular atrophy of the femoral region and hyperextension of hindlimbs. The dog had positive serum IFAT titers to Neospora caninum, but a negative titer in the cerebrospinal fluid (CSF). N. caninum-specific DNA was amplified from the CSF using a semi-nested polymerase chain reaction assay. Clusters of protozoa in biopsied muscle fibers were subsequently confirmed as N. caninum tachyzoites by immunohistochemical examination. Early recognition and treatment are necessary for effective recovery of clinical canine neosporosis, but antemortem diagnosis is difficult. We suggest that the detection of parasite deoxyribonucleic acid in the CSF is a useful antemortem diagnostic method in facilitating treatment of this disease.

引用

页码：1051 / 1055

页数：5

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